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Science 20 March 1987:
Vol. 235. no. 4795, pp. 1489 - 1492
DOI: 10.1126/science.3547651
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Articles

Science, Vol 235, Issue 4795, 1489-1492
Copyright © 1987 by American Association for the Advancement of Science

articles

Molecular cloning and expression of a human B-cell growth factor gene in Escherichia coli

S Sharma, S Mehta, J Morgan, and A Maizel

A human B-cell growth factor (BCGF) (12 kilodaltons) supports the clonal proliferation of B lymphocytes. A clone was isolated that contained the proper structural sequence to encode biologically active, 12-kilodalton BCGF in Escherichia coli and to hybridize to a specific messenger RNA, identified by in vitro translation in Xenopus laevis oocytes. A relatively hydrophobic region of 18 amino acids was found at the amino terminal of the 124-amino acid-long polypeptide. The carboxyl terminal is composed of at least 32 amino acids that are derived from nucleotide sequences bearing significant homology to the Alu repeat family.


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Science. ISSN 0036-8075 (print), 1095-9203 (online)