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Science 13 March 1987:
Vol. 235. no. 4794, pp. 1392 - 1394
DOI: 10.1126/science.3029869
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Articles

Science, Vol 235, Issue 4794, 1392-1394
Copyright © 1987 by American Association for the Advancement of Science

articles

Transposition of gram-positive transposon Tn916 in Acholeplasma laidlawii and Mycoplasma pulmonis

K Dybvig and GH Cassell

Mycoplasma genetics has been limited by a lack of genetic tools such as selectable markers, methods to transfer DNA, and suitable vectors for cloning. Studies were undertaken to examine the potential of using the streptococcal transposon Tn916 as a mycoplasma genetic tool. The Escherichia coli plasmid pAM120, which contains Tn916, was transformed into Acholeplasma laidlawii and Mycoplasma pulmonis. Transposition of Tn916 into the mycoplasma chromosome apparently occurred by an excision-insertion mechanism. This example shows that newly introduced DNA from other bacteria can be successfully expressed in mycoplasma and that Tn916 should serve as a powerful genetic tool for the study of mycoplasmas.


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Avoidance of the Host Immune System through Phase Variation in Mycoplasma pulmonis.
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Gene Transfer in Mycoplasma pulmonis.
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The complete genome sequence of the murine respiratory pathogen Mycoplasma pulmonis.
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Molecular Biology and Pathogenicity of Mycoplasmas.
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A family of phase-variable restriction enzymes with differing specificities generated by high-frequency gene rearrangements.
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