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Science 13 March 1987:
Vol. 235. no. 4794, pp. 1383 - 1387
DOI: 10.1126/science.3103216
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Articles

Science, Vol 235, Issue 4794, 1383-1387
Copyright © 1987 by American Association for the Advancement of Science

articles

Regulation in vitro of metallothionein gene binding factors

C Seguin and DH Hamer

Mouse nuclear factors that bind to an upstream metal regulatory element of the mouse metallothionein-I gene have been identified by DNA footprinting and oligonucleotide band shift assays. The formation of complexes at this site can be activated 20- to 40-fold by the vitro addition of ionic cadmium. The activation reaction is rapid, reversible by a metal chelator, and may involve multiple proteins. These results suggest that the initial step in cadmium detoxification is an interaction between the metal and nuclear DNA-binding factors leading to an increase in metallothionein gene transcription. The ability to observe metal activation in vitro makes this a powerful system to study the biochemistry of eukaryotic gene regulation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nitric oxide-induced changes in intracellular zinc homeostasis are mediated by metallothionein/thionein.
C. M. St. Croix, K. J. Wasserloos, K. E. Dineley, I. J. Reynolds, E. S. Levitan, and B. R. Pitt (2002)
Am J Physiol Lung Cell Mol Physiol 282, L185-L192
   Abstract »    Full Text »    PDF »
Zinc and Cadmium Can Promote Rapid Nuclear Translocation of Metal Response Element-binding Transcription Factor-1.
I. V. Smirnova, D. C. Bittel, R. Ravindra, H. Jiang, and G. K. Andrews (2000)
J. Biol. Chem. 275, 9377-9384
   Abstract »    Full Text »    PDF »
Constitutive and metal-inducible protein:DNA interactions at the mouse metallothionein I promoter examined by in vivo and in vitro footprinting..
P R Mueller, S J Salser, and B Wold (1988)
Genes & Dev. 2, 412-427
   Abstract »    PDF »


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