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Science 27 February 1987:
Vol. 235. no. 4792, pp. 1059 - 1062
DOI: 10.1126/science.2434994
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Articles

Science, Vol 235, Issue 4792, 1059-1062
Copyright © 1987 by American Association for the Advancement of Science

articles

Construction of synthetic immunogen: use of new T-helper epitope on malaria circumsporozoite protein

MF Good, WL Maloy, MN Lunde, H Margalit, JL Cornette, GL Smith, B Moss, LH Miller, and JA Berzofsky

The circumsporozoite (CS) protein of Plasmodium falciparum is the focus of intense efforts to develop an antisporozoite malaria vaccine. Localization of sites for T-cell recognition on this molecule is critical for vaccine design. By using an algorithm designed to predict T-cell sites and a large panel of H-2 congenic mice, a major nonrepetitive T-cell site was located. When a synthetic peptide corresponding to this site was covalently linked to the major B-cell site on the molecule, an immunogen capable of eliciting a high-titer antibody response was formed. This peptide sequence could prime helper T cells for a secondary response to the intact CS protein. The new helper T-cell site is located outside the repetitive region of the CS protein and appears to be the immunodominant T site on the molecule. This approach should be useful in the rational design and construction of vaccines.


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A Contraceptive Peptide Vaccine Targeting Sulfated Glycoprotein ZP2 of the Mouse Zona Pellucida.
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Epitope-Specific Antibody and Suppression of Autoantibody Responses Against a Hybrid Self Protein.
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Induction of Protective Immune Responses by Immunization with Linear Multiepitope Peptides Based on Conserved Sequences from Plasmodium falciparum Antigens.
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A Role for the Disintegrin Domain of Cyritestin, a Sperm Surface Protein Belonging to the ADAM Family, in Mouse Sperm-Egg Plasma Membrane Adhesion and Fusion.
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J. Cell Biol. 137, 105-112
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Anchoring of an Immunogenic Plasmodium falciparum Circumsporozoite Protein on the Surface of Dictyostelium discoideum.
C. D. Reymond, C. Beghdadi-Rais, M. Roggero, E. A. Duarte, C. Desponds, M. Bernard, D. Groux, H. Matile, C. Bron, G. Corradin, et al. (1995)
J. Biol. Chem. 270, 12941-12947
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Vaccination with a synthetic zona pellucida peptide produces long-term contraception in female mice.
S. Millar, S. Chamow, A. Baur, C Oliver, F Robey, and J Dean (1989)
Science 246, 935-938
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Limited immunological recognition of critical malaria vaccine candidate antigens.
M. Good, L. Miller, S Kumar, I. Quakyi, D Keister, J. Adams, B Moss, J. Berzofsky, and R Carter (1988)
Science 242, 574-577
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Proteosome-lipopeptide vaccines: enhancement of immunogenicity for malaria CS peptides.
G. Lowell, W. Ballou, L. Smith, R. Wirtz, W. Zollinger, and W. Hockmeyer (1988)
Science 240, 800-802
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Oral Salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria.
J. Sadoff, W. Ballou, L. Baron, W. Majarian, R. Brey, W. Hockmeyer, J. Young, S. Cryz, J Ou, G. Lowell, et al. (1988)
Science 240, 336-338
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