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Science 27 February 1987:
Vol. 235. no. 4792, pp. 1040 - 1043
DOI: 10.1126/science.3547646
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Articles

Science, Vol 235, Issue 4792, 1040-1043
Copyright © 1987 by American Association for the Advancement of Science

articles

Encystation and expression of cyst antigens by Giardia lamblia in vitro

FD Gillin, DS Reiner, MJ Gault, H Douglas, S Das, A Wunderlich, and JF Sauch

The cyst form of Giardia lamblia is responsible for transmission of giardiasis, a common waterborne intestinal disease. In these studies, encystation of Giardia lamblia in vitro was demonstrated by morphologic, immunologic, and biochemical criteria. In the suckling mouse model, the jejunum was shown to be a major site of encystation of the parasite. Small intestinal factors were therefore tested as stimuli of encystation. An antiserum that reacted with cysts, but not with cultured trophozoites was raised in rabbits and used as a sensitive probe for differentiation in vitro. Cultured trophozoites that were exposed to bile salts showed a more than 20-fold increase in the number of oval, refractile cells that reacted strongly with anticyst antibodies, and in the expression of major cyst antigens. Exposure to primary bile salts resulted in higher levels of encystation than exposure to secondary bile salts. These studies will aid in understanding the differentiation of an important protozoan pathogen.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Identification of the Major Cysteine Protease of Giardia and Its Role in Encystation.
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Mast Cell-Mediated Changes in Smooth Muscle Contractility during Mouse Giardiasis.
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A Novel ARID/Bright-like Protein Involved in Transcriptional Activation of Cyst Wall Protein 1 Gene in Giardia lamblia.
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Oral Immunization of BALB/c Mice with Giardia duodenalis Recombinant Cyst Wall Protein Inhibits Shedding of Cysts.
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Mining the Giardia lamblia Genome for New Cyst Wall Proteins.
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The Activity of a Developmentally Regulated Cysteine Proteinase Is Required for Cyst Wall Formation in the Primitive Eukaryote Giardia lamblia.
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Biology of Giardia lamblia.
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Identification of a Novel Giardia lamblia Cyst Wall Protein with Leucine-rich Repeats.
H. D. Luján, M. R. Mowatt, J. T. Conrad, B. Bowers, and T. E. Nash (1995)
J. Biol. Chem. 270, 29307-29313
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