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Science 30 January 1987:
Vol. 235. no. 4788, pp. 591 - 593
DOI: 10.1126/science.3810159
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Articles

Science, Vol 235, Issue 4788, 591-593
Copyright © 1987 by American Association for the Advancement of Science

articles

A G1 glycoprotein epitope of La Crosse virus: a determinant of infection of Aedes triseriatus

DR Sundin, BJ Beaty, N Nathanson, and F Gonzalez-Scarano

Arthropod-borne viruses (arboviruses) have specific vector-vertebrate host cycles in nature. The molecular basis of restriction of virus replication to a very limited number of vector species is unknown, but the present study suggests that viral attachment proteins are important determinants of vector-virus interactions. The principal vector of La Crosse (LAC) virus is the mosquito Aedes triseriatus, and LAC virus efficiently infects the mosquito when ingested. However, a variant (V22) of LAC virus, which was selected by growing the virus in the presence of a monoclonal antibody, was markedly restricted in its ability to infect Ae. triseriatus when it was ingested. Only 15% of the mosquitoes that ingested V22 became infected and 5% of these developed disseminated infections. In contrast, 89% of the mosquitoes that ingested LAC became infected and 74% developed disseminated infections. When V22 was passed three times in mosquitoes by feeding, a revertant virus, V22M3, was obtained that infected 85% of Ae. triseriatus ingesting this virus. In addition, V22M3 regained the antigenic phenotype and fusion capability of the parent LAC virus. These results suggest that the specificity of LAC virus-vector interactions is markedly influenced by the efficiency of the fusion function of the G1 envelope glycoprotein operating at the midgut level in the arthropod vector.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
MANIPULATION OF THE YELLOW FEVER VIRUS NON-STRUCTURAL GENES 2A AND 4B AND THE 3'NON-CODING REGION TO EVALUATE GENETIC DETERMINANTS OF VIRAL DISSEMINATION FROM THE AEDES AEGYPTI MIDGUT.
K. L. McELROY, K. A. TSETSARKIN, D. L. VANLANDINGHAM, and S. HIGGS (2006)
Am J Trop Med Hyg 75, 1158-1164
   Abstract »    Full Text »    PDF »
Role of the yellow fever virus structural protein genes in viral dissemination from the Aedes aegypti mosquito midgut..
K. L. McElroy, K. A. Tsetsarkin, D. L. Vanlandingham, and S. Higgs (2006)
J. Gen. Virol. 87, 2993-3001
   Abstract »    Full Text »    PDF »
Thrips and tospoviruses come of age: Mapping determinants of insect transmission.
D. E. Ullman, A. E. Whitfield, and T. L. German (2005)
PNAS 102, 4931-4932
   Full Text »    PDF »
Expression and Characterization of a Soluble Form of Tomato Spotted Wilt Virus Glycoprotein GN.
A. E. Whitfield, D. E. Ullman, and T. L. German (2004)
J. Virol. 78, 13197-13206
   Abstract »    Full Text »    PDF »
Vector Infection Determinants of Venezuelan Equine Encephalitis Virus Reside within the E2 Envelope Glycoprotein.
A. C. Brault, A. M. Powers, and S. C. Weaver (2002)
J. Virol. 76, 6387-6392
   Abstract »    Full Text »    PDF »
Bunyavirus superinfection and segment reassortment in transovarially infected mosquitoes.
M. K. Borucki, L. J. Chandler, B. M. Parker, C. D. Blair, and B. J. Beaty (1999)
J. Gen. Virol. 80, 3173-3179
   Abstract »    Full Text »
Topical Review: La Crosse and Other Forms of California Encephalitis.
R. S. Rust, W. H. Thompson, C. G. Matthews, B. J. Beaty, and R. W.M. Chun (1999)
J Child Neurol 14, 1-14
   Abstract »    PDF »


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