Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Focus on Europe

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 30 January 1987:
Vol. 235. no. 4788, pp. 587 - 591
DOI: 10.1126/science.235.4788.587
Prev | Table of Contents | Next

Articles

Site-Specific Nick in the T-DNA Border Sequence as a Result of Agrobacterium vir Gene Expression

KAN WANG 1, SCOTT E. STACHEL 2, BENEDIKT TIMMERMAN 1, MARC VAN MONTAGU 1, and PATRICIA C. ZAMBRYSKI 2

1 Laboratorium voor Genetica, Rijksuniversiteit Gent, B-9000 Gent, Belgium.
2 Division of Molecular Plant Biology, Hilgard Hall, University of California, Berkeley, CA 94720.

The T-DNA transfer process of Agrobacterium tumefaciens is activated by the induction of the expression of the Ti plasmid virulence (vir) loci by plant signal molecules such as acetosyringone. The vir gene products act in trans to mobilize the T-DNA element from the bacterial Ti plasmid. The T-DNA is bounded by 25—base pair direct repeat sequences, which are the only sequences on the element essential for transfer. Thus, specific reactions must occur at the border sites to generate a transferable T-DNA copy. The T-DNA border sequences were shown in this study to be specifically nicked after vir gene activation. Border nicks were detected on the bottom strand just after the third or fourth base (± one or two nucleotides) of the 25—base pair transferpromoting sequence. Naturally occurring and base-substituted derivatives of the 25—base pair sequences are effective substrates for acetosyringone-induced border cleavage, whereas derivatives carrying only the first 15 or last 19 base pairs of the 25—base pair sequence are not. Site-specific border cleavages occur within 12 hours after acetosyringone induction and probably represent an early step in the T-DNA transfer process.

Submitted on August 11, 1986
Accepted on December 11, 1986


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
T-DNA Binary Vectors and Systems.
L.-Y. Lee and S. B. Gelvin (2008)
Plant Physiology 146, 325-332
   Full Text »    PDF »
Three-dimensional Reconstruction of Agrobacterium VirE2 Protein with Single-stranded DNA.
A. Abu-Arish, D. Frenkiel-Krispin, T. Fricke, T. Tzfira, V. Citovsky, S. G. Wolf, and M. Elbaum (2004)
J. Biol. Chem. 279, 25359-25363
   Abstract »    Full Text »    PDF »
The Agrobacterium-Plant Cell Interaction. Taking Biology Lessons from a Bug.
T. Tzfira and V. Citovsky (2003)
Plant Physiology 133, 943-947
   Full Text »    PDF »
Site-Specific Integration of Agrobacterium tumefaciens T-DNA via Double-Stranded Intermediates.
T. Tzfira, L. R. Frankman, M. Vaidya, and V. Citovsky (2003)
Plant Physiology 133, 1011-1023
   Abstract »    Full Text »    PDF »
Agrobacterium-Mediated Plant Transformation: the Biology behind the "Gene-Jockeying" Tool.
S. B. Gelvin (2003)
Microbiol. Mol. Biol. Rev. 67, 16-37
   Abstract »    Full Text »    PDF »
"Agrolistic" transformation of plant cells: Integration of T-strands generated in planta.
G. Hansen and M.-D. Chilton (1996)
PNAS 93, 14978-14983
   Abstract »    Full Text »    PDF »
Single-Stranded DNA Binding Protein Encoded by the virE Locus of Agrobacterium tumefaciens.
V. CITOVSKY, G. DE VOS, and P. ZAMBRYSKI (1988)
Science 240, 501-504
   Abstract »    PDF »
Transgenic Plants as Tools to Study the Molecular Organization of Plant Genes.
J. ST. SCHELL (1987)
Science 237, 1176-1183
   Abstract »    PDF »
Genetic transformation of HeLa cells by Agrobacterium.
T. Kunik, T. Tzfira, Y. Kapulnik, Y. Gafni, C. Dingwall, and V. Citovsky (2001)
PNAS 98, 1871-1876
   Abstract »    Full Text »    PDF »


ADVERTISEMENT
Click Me!

ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)