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Science 30 January 1987:
Vol. 235. no. 4788, pp. 571 - 574
DOI: 10.1126/science.3810156
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Articles

Science, Vol 235, Issue 4788, 571-574
Copyright © 1987 by American Association for the Advancement of Science

articles

Structures of two thermolysin-inhibitor complexes that differ by a single hydrogen bond

DE Tronrud, HM Holden, and BW Matthews

The mode of binding to thermolysin of the ester analog Cbz-GlyP-(O)-Leu-Leu has been determined by x-ray crystallography and shown to be virtually identical (maximum difference 0.2 angstrom) with the corresponding peptide analog Cbz-GlyP-(NH)-Leu-Leu. The two inhibitors provide a matched pair of enzyme-inhibitor complexes that differ by 4.1 kilocalories per mole in intrinsic binding energy but are essentially identical except for the presence or absence of a specific hydrogen bond.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Critical Contribution of Aromatic Rings to Specific Recognition of Polyether Rings: THE CASE OF CIGUATOXIN CTX3C-ABC AND ITS SPECIFIC ANTIBODY 1C49.
K. Tsumoto, A. Yokota, Y. Tanaka, M. Ui, T. Tsumuraya, I. Fujii, I. Kumagai, Y. Nagumo, H. Oguri, M. Inoue, et al. (2008)
J. Biol. Chem. 283, 12259-12266
   Abstract »    Full Text »    PDF »
Structural Consequences of Mutations in Interfacial Tyr Residues of a Protein Antigen-Antibody Complex: THE CASE OF HyHEL-10-HEL.
M. Shiroishi, K. Tsumoto, Y. Tanaka, A. Yokota, T. Nakanishi, H. Kondo, and I. Kumagai (2007)
J. Biol. Chem. 282, 6783-6791
   Abstract »    Full Text »    PDF »
Enzymatic catalysts in organic synthesis.
C. Wong (1989)
Science 244, 1145-1152
   Abstract »    PDF »
Evaluation of intrinsic binding energy from a hydrogen bonding group in an enzyme inhibitor.
P. Bartlett and C. Marlowe (1987)
Science 235, 569-571
   Abstract »    PDF »
Calculation of the relative change in binding free energy of a protein-inhibitor complex.
P. Bash, U. Singh, F. Brown, R Langridge, and P. Kollman (1987)
Science 235, 574-576
   Abstract »    PDF »
Phosphorus-containing Peptide Analogs as Peptidase Inhibitors.
P.A. Bartlett, C.K. Marlowe, P.P. Giannousis, and J.E. Hanson (1987)
Cold Spring Harb Symp Quant Biol 52, 83-90
   Abstract »    PDF »
Engineering of Antibodies with a Known Three-dimensional Structure.
A. Pluckthun, R. Glockshuber, I. Pfitzinger, A. Skerra, and J. Stadlmuller (1987)
Cold Spring Harb Symp Quant Biol 52, 105-112
   Abstract »    PDF »


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