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Science 23 January 1987:
Vol. 235. no. 4787, pp. 456 - 458
DOI: 10.1126/science.3099390
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Articles

Science, Vol 235, Issue 4787, 456-458
Copyright © 1987 by American Association for the Advancement of Science

articles

In situ detection of beta-galactosidase in lenses of transgenic mice with a gamma-crystallin/lacZ gene

DR Goring, J Rossant, S Clapoff, ML Breitman, and LC Tsui

Transgenic mice carrying the gamma 2-crystallin promoter fused to the coding region of the bacterial lacZ gene were generated. The offspring of three founder mice expressed high levels of the enzyme solely in the central nuclear fiber cells of the lens as measured by an in situ assay for the detection of beta-galactosidase activity. These results suggest that gamma 2-crystallin sequences between -759 to +45 contain essential information required for appropriate tissue-specific and temporal regulation of the mouse gamma 2-crystallin gene. In a broader context, this study also demonstrates the utility of beta-galactosidase hybrid gene constructs for monitoring the activity of gene regulatory elements in transgenic mice.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Whole Mount Enzyme Histochemistry as a Rapid Screen at Necropsy for Expression of {beta}-Galactosidase (LacZ)-Bearing Transgenes: Considerations for Separating Specific LacZ Activity from Nonspecific (Endogenous) Galactosidase Activity.
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The Murine Cyp1a1 Gene Is Expressed in a Restricted Spatial and Temporal Pattern during Embryonic Development.
S. J. Campbell, C. J. Henderson, D. C. Anthony, D. Davidson, A. J. Clark, and C. R. Wolf (2005)
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Insertion of a Pax6 Consensus Binding Site into the {alpha}A-Crystallin Promoter Acts as a Lens Epithelial Cell Enhancer in Transgenic Mice.
H. Zhao, Y. Yang, C. M. Rizo, P. A. Overbeek, and M. L. Robinson (2004)
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Transgenic Mouse Models of Human CYP3A4 Gene Regulation.
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The development of Xenopus tropicalis transgenic lines and their use in studying lens developmental timing in living embryos.
M. Offield, N Hirsch, and R. Grainger (2000)
Development 127, 1789-1797
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Retinal Expression of {gamma}-Crystallins in the Mouse.
S. E. Jones, C. Jomary, J. Grist, J. Makwana, and M. J. Neal (1999)
Invest. Ophthalmol. Vis. Sci. 40, 3017-3020
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Enhanced In Situ Detection of ß-Glucuronidase Activity in Murine Tissue.
G. C. Kopen, D. J. Prockop, and D. G. Phinney (1999)
J. Histochem. Cytochem. 47, 965-968
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An everted repeat mediates retinoic acid induction of the gamma F-crystallin gene: evidence of a direct role for retinoids in lens development..
M Tini, G Otulakowski, M L Breitman, L C Tsui, and V Giguere (1993)
Genes & Dev. 7, 295-307
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Defective lens fiber differentiation and pancreatic tumorigenesis caused by ectopic expression of the cellular retinoic acid-binding protein I.
A. Perez-Castro, V. Tran, and M. Nguyen-Huu (1993)
Development 119, 363-375
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Expression of Pax-3- and neuroectoderm-inducing activities during differentiation of P19 embryonal carcinoma cells.
S. Pruitt (1992)
Development 116, 573-583
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Transgenic animals.
R Jaenisch (1988)
Science 240, 1468-1474
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Genetic ablation: targeted expression of a toxin gene causes microphthalmia in transgenic mice.
M. Breitman, S Clapoff, J Rossant, L. Tsui, L. Glode, I. Maxwell, and A Bernstein (1987)
Science 238, 1563-1565
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Expression and assembly of a human neurofilament protein in transgenic mice provide a novel neuronal marking system..
J P Julien, I Tretjakoff, L Beaudet, and A Peterson (1987)
Genes & Dev. 1, 1085-1095
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Lens-specific enhancer in the third intron regulates expression of the chicken delta 1-crystallin gene..
S Hayashi, K Goto, T S Okada, and H Kondoh (1987)
Genes & Dev. 1, 818-828
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The Upstream Region of the Rpe65 Gene Confers Retinal Pigment Epithelium-specific Expression in Vivo and in Vitro and Contains Critical Octamer and E-box Binding Sites.
A. Boulanger, S. Liu, A. A. Henningsgaard, S. Yu, and T. M. Redmond (2000)
J. Biol. Chem. 275, 31274-31282
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