Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 16 January 1987:
Vol. 235. no. 4786, pp. 325 - 328
DOI: 10.1126/science.3798114
Prev | Table of Contents | Next

Articles

Science, Vol 235, Issue 4786, 325-328
Copyright © 1987 by American Association for the Advancement of Science

articles

Cellular and subcellular heterogeneity of [Ca2+]i in single heart cells revealed by fura-2

WG Wier, MB Cannell, Berlin JR, E Marban, and WJ Lederer

Digital imaging of calcium indicator signals (fura-2 fluorescence) from single cardiac cells has revealed different subcellular patterns of cytoplasmic calcium ion concentration ([Ca2+]i) that are associated with different types of cellular appearance and behavior. In any population of enzymatically isolated rat heart cells, there are mechanically quiescent cells in which [Ca2+]i is spatially uniform, constant over time, and relatively low; spontaneously contracting cells, which have an increased [Ca2+]i, but in which the spatial uniformity of [Ca2+]i is interrupted periodically by spontaneous propagating waves of high [Ca2+]i; and cells that are hypercontracted (rounded up) and that have higher levels of [Ca2+]i than the other two types. The observed cellular and subcellular heterogeneity of [Ca2+]i in isolated cells indicates that experiments performed on suspensions of cells should be interpreted with caution. The spontaneous [Ca2+]i fluctuations previously observed without spatial resolution in multicellular preparations may actually be inhomogeneous at the subcellular level.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Calcium Sparks.
H. Cheng and W. J. Lederer (2008)
Physiol Rev 88, 1491-1545
   Abstract »    Full Text »    PDF »
The sarcoplasmic reticulum and arrhythmogenic calcium release.
L. A. Venetucci, A. W. Trafford, S. C. O'Neill, and D. A. Eisner (2008)
Cardiovasc Res 77, 285-292
   Abstract »    Full Text »    PDF »
Calcium and Arrhythmogenesis.
H. E. D. J. ter Keurs and P. A. Boyden (2007)
Physiol Rev 87, 457-506
   Abstract »    Full Text »    PDF »
Calcium waves driven by "sensitization" wave-fronts.
M. Keller, J. P.Y. Kao, M. Egger, and E. Niggli (2007)
Cardiovasc Res 74, 39-45
   Abstract »    Full Text »    PDF »
The Cardiac Sarcoplasmic Reticulum: Filled With Ca2+ and Surprises.
E. Niggli (2007)
Circ. Res. 100, 5-6
   Full Text »    PDF »
Increasing Ryanodine Receptor Open Probability Alone Does Not Produce Arrhythmogenic Calcium Waves: Threshold Sarcoplasmic Reticulum Calcium Content Is Required.
L. A. Venetucci, A. W. Trafford, and D. A. Eisner (2007)
Circ. Res. 100, 105-111
   Abstract »    Full Text »    PDF »
Reducing Ryanodine Receptor Open Probability as a Means to Abolish Spontaneous Ca2+ Release and Increase Ca2+ Transient Amplitude in Adult Ventricular Myocytes.
L.A. Venetucci, A.W. Trafford, M.E. Diaz, S.C. O'Neill, and D.A. Eisner (2006)
Circ. Res. 98, 1299-1305
   Abstract »    Full Text »    PDF »
Paradoxical SR Ca2+ release in guinea-pig cardiac myocytes after {beta}-adrenergic stimulation revealed by two-photon photolysis of caged Ca2+.
N. Lindegger and E. Niggli (2005)
J. Physiol. 565, 801-813
   Abstract »    Full Text »    PDF »
Modulation of cytosolic and intra-sarcoplasmic reticulum calcium waves by calsequestrin in rat cardiac myocytes.
Z. Kubalova, I. Gyorke, R. Terentyeva, S. Viatchenko-Karpinski, D. Terentyev, S. C Williams, and S. Gyorke (2004)
J. Physiol. 561, 515-524
   Abstract »    Full Text »    PDF »
Comparison of Ca2+ release and uptake characteristics of the sarcoplasmic reticulum in isolated horse and rabbit cardiomyocytes.
C. M. Loughrey, G. L. Smith, and K. E. MacEachern (2004)
Am J Physiol Heart Circ Physiol 287, H1149-H1159
   Abstract »    Full Text »    PDF »
Interplay between SERCA and sarcolemmal Ca2+ efflux pathways controls spontaneous release of Ca2+ from the sarcoplasmic reticulum in rat ventricular myocytes.
S. C. O'Neill, L. Miller, R. Hinch, and D. A. Eisner (2004)
J. Physiol. 559, 121-128
   Abstract »    Full Text »    PDF »
Imaging Microdomain Ca2+ in Muscle Cells.
S.-Q. Wang, C. Wei, G. Zhao, D. X.P. Brochet, J. Shen, L.-S. Song, W. Wang, D. Yang, and H. Cheng (2004)
Circ. Res. 94, 1011-1022
   Abstract »    Full Text »    PDF »
A model of graded calcium release and L-type Ca2+ channel inactivation in cardiac muscle.
V. E. Bondarenko, G. C. L. Bett, and R. L. Rasmusson (2004)
Am J Physiol Heart Circ Physiol 286, H1154-H1169
   Abstract »    Full Text »    PDF »
The Voltage-sensitive Release Mechanism of Excitation Contraction Coupling in Rabbit Cardiac Muscle Is Explained by Calcium-induced Calcium Release.
H. Griffiths and K.T. MacLeod (2003)
J. Gen. Physiol. 121, 353-373
   Abstract »    Full Text »    PDF »
Regulation of junctional and non-junctional sarcoplasmic reticulum calcium release in excitation-contraction coupling in cat atrial myocytes.
K. A Sheehan and L. A Blatter (2003)
J. Physiol. 546, 119-135
   Abstract »    Full Text »    PDF »
A comparison of the effects of ATP and tetracaine on spontaneous Ca2+ release from rat permeabilised cardiac myocytes.
G L Smith and S C O'Neill (2001)
J. Physiol. 534, 37-47
   Abstract »    Full Text »    PDF »
Intra-uterine growth retardation results in increased cardiac arrhythmias and raised diastolic blood pressure in adult rats.
X.W Hu, A Levy, E.J Hart, L.A Nolan, G.R Dalton, and A.J Levi (2000)
Cardiovasc Res 48, 233-243
   Abstract »    Full Text »    PDF »
No Role for the Ryanodine Receptor in Regulating Cardiac Contraction?.
D. A. Eisner and A. W. Trafford (2000)
Physiology 15, 275-279
   Abstract »    Full Text »    PDF »
Progress in the understanding of cardiac early afterdepolarizations and torsades de pointes: time to revise current concepts.
P. G.A. Volders, M. A. Vos, B. Szabo, K. R. Sipido, S.H.M. de Groot, A. P.M. Gorgels, H. J.J. Wellens, and R. Lazzara (2000)
Cardiovasc Res 46, 376-392
   Full Text »    PDF »
Three Distinct Types of Ca2+ Waves in Langendorff-Perfused Rat Heart Revealed by Real-Time Confocal Microscopy.
T. Kaneko, H. Tanaka, M. Oyamada, S. Kawata, and T. Takamatsu (2000)
Circ. Res. 86, 1093-1099
   Abstract »    Full Text »    PDF »
Inhibition of sarcoplasmic reticulum function by polyunsaturated fatty acids in intact, isolated myocytes from rat ventricular muscle.
N Negretti, M R Perez, D Walker, and S C O'Neill (2000)
J. Physiol. 523, 367-375
   Abstract »    Full Text »    PDF »
Ca2+ sparks and Ca2+ waves in saponin-permeabilized rat ventricular myocytes.
V. Lukyanenko and S. Gyorke (1999)
J. Physiol. 521, 575-585
   Abstract »    Full Text »    PDF »
Ca2+ Sparks in Cardiac Muscle: Is There Life Without Them?.
E. Niggli (1999)
Physiology 14, 129-134
   Abstract »    Full Text »    PDF »
The role of luminal Ca2+ in the generation of Ca2+ waves in rat ventricular myocytes.
V. Lukyanenko, S. Subramanian, I. Gyorke, T. F Wiesner, and S. Gyorke (1999)
J. Physiol. 518, 173-186
   Abstract »    Full Text »    PDF »
Damage-induced arrhythmias: reversal of excitation-contraction coupling.
H. E.D.J ter Keurs, Y. M. Zhang, and M. Miura (1998)
Cardiovasc Res 40, 444-455
   Full Text »    PDF »
Intercellular Ca2+ waves in rat heart muscle.
C. Lamont, P. W Luther, C W. Balke, and W G. Wier (1998)
J. Physiol. 512, 669-676
   Abstract »    Full Text »    PDF »
Direct measurement of SR release flux by tracking 'Ca2+ spikes' in rat cardiac myocytes.
L.-S. Song, J. S K Sham, M. D Stern, E. G Lakatta, and H. Cheng (1998)
J. Physiol. 512, 677-691
   Abstract »    Full Text »    PDF »
Cultured adult cardiac myocytes: Future applications, culture methods, morphological and electrophysiological properties.
J. S Mitcheson, J. C Hancox, and A. J Levi (1998)
Cardiovasc Res 39, 280-300
   Abstract »    Full Text »    PDF »
Ca2+ `Sparks' and Waves in Intact Ventricular Muscle Resolved by Confocal Imaging.
W. G. Wier, H. E. D. J. t. Keurs, E. Marban, W. D. Gao, and C. W. Balke (1997)
Circ. Res. 81, 462-469
   Abstract »    Full Text »
Triggered Propagated Contractions in Rat Cardiac Trabeculae: Inhibition by Octanol and Heptanol.
Y. M. Zhang, M. Miura, and H.E.D.J. ter Keurs (1996)
Circ. Res. 79, 1077-1085
   Abstract »    Full Text »
POTASSIUM CHANNEL OPENERS PREVENT POTASSIUM-INDUCED CALCIUM LOADING OF CARDIAC CELLS: POSSIBLE IMPLICATIONS IN CARDIOPLEGIA.
J. R. Lopez, R. Jahangir, A. Jahangir, W. K. Shen, and A. Terzic (1996)
J. Thorac. Cardiovasc. Surg. 112, 820-831
   Abstract »    Full Text »
Variability of Spontaneous Ca2+ Release Between Different Rat Ventricular Myocytes Is Correlated With Na+-Ca2+ Exchange and [Na+]i.
M.E. Diaz, S.J. Cook, J.P. Chamunorwa, A.W. Trafford, M.K. Lancaster, S.C. O'Neill, and D.A. Eisner (1996)
Circ. Res. 78, 857-862
   Abstract »    Full Text »
Effects of Action Potential Duration on Excitation-Contraction Coupling in Rat Ventricular Myocytes : Action Potential Voltage-Clamp Measurements.
R. A. Bouchard, R. B. Clark, and W. R. Giles (1995)
Circ. Res. 76, 790-801
   Abstract »    Full Text »
Calcium sparks: elementary events underlying excitation-contraction coupling in heart muscle.
H Cheng, W. Lederer, and M. Cannell (1993)
Science 262, 740-744
   Abstract »    PDF »
A spatial-temporal model of cell activation.
D. Alkon and H Rasmussen (1988)
Science 239, 998-1005
   Abstract »    PDF »
Agonist-induced Calcium Oscillations in Depolarized Fibroblasts and Their Manipulation by Photoreleased Ins(l,4,5)P3, and Ca++, and Ca++ Buffer.
A.T. Harootunian, J.P.Y. Kao, and R.Y. Tsien (1988)
Cold Spring Harb Symp Quant Biol 53, 935-943
   Abstract »    PDF »
Sodium-calcium exchange in heart: membrane currents and changes in [Ca2+]i.
L Barcenas-Ruiz, D. Beuckelmann, and W. Wier (1987)
Science 238, 1720-1722
   Abstract »    PDF »
Effect of membrane potential changes on the calcium transient in single rat cardiac muscle cells.
M. Cannell, Berlin JR, and W. Lederer (1987)
Science 238, 1419-1423
   Abstract »    PDF »
Contribution of Ryanodine Receptor Subtype 3 to Ca2+ Responses in Ca2+-overloaded Cultured Rat Portal Vein Myocytes.
J. Mironneau, F. Coussin, L. H. Jeyakumar, S. Fleischer, C. Mironneau, and N. Macrez (2001)
J. Biol. Chem. 276, 11257-11264
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)