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Science 16 January 1987:
Vol. 235. no. 4786, pp. 312 - 317
DOI: 10.1126/science.3541205
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Articles

Science, Vol 235, Issue 4786, 312-317
Copyright © 1987 by American Association for the Advancement of Science

articles

Many random sequences functionally replace the secretion signal sequence of yeast invertase

CA Kaiser, D Preuss, P Grisafi, and D Botstein

In the process of protein secretion, amino-terminal signal sequences are key recognition elements; however, the relation between the primary sequence of an amino-terminal peptide and its ability to function as an export signal remains obscure. The limits of variation permitted for functional signal sequences were determined by replacement of the normal signal sequence of Saccharomyces cerevisiae invertase with essentially random peptide sequences. Since about one-fifth of these sequences can function as an export signal the specificity with which signal sequences are recognized must be very low.


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