Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 19 December 1986:
Vol. 234. no. 4783, pp. 1552 - 1557
DOI: 10.1126/science.3491428
Prev | Table of Contents | Next

Articles

Science, Vol 234, Issue 4783, 1552-1557
Copyright © 1986 by American Association for the Advancement of Science

articles

Two different cis-active elements transfer the transcriptional effects of both EGF and phorbol esters

HP Elsholtz, HJ Mangalam, E Potter, VR Albert, S Supowit, RM Evans, and MG Rosenfeld

Short cis-active sequences of the rat prolactin or Moloney murine leukemia virus genes transfer transcriptional regulation by both epidermal growth factor and phorbol esters to fusion genes. These sequences act in a position- and orientation-independent manner. Competitive binding analyses with nuclear extracts from stimulated and unstimulated cells suggest that different trans-acting factors associate with the regulatory sequence of each gene. A model is proposed suggesting that both epidermal growth factor and phorbol esters stimulate the transcription of responsive genes via discrete classes of hormone-dependent, enhancer-like elements that bind different trans-acting factors, even in the absence of hormone stimulation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Role of Calcium-Calmodulin-Dependent Protein Kinase Cascade in Thyrotropin (TSH)-Releasing Hormone Induction of TSH and Prolactin Gene Expression.
K. Murao, H. Imachi, W. M. Cao, X. Yu, H. Tokumitsu, H. Inuzuka, N. C. W. Wong, M. A. Shupnik, R. Kobayashi, and T. Ishida (2004)
Endocrinology 145, 4846-4852
   Abstract »    Full Text »    PDF »
Cocaine Produces Cardiac Hypertrophy by Protein Kinase C Dependent Mechanisms.
R. J. Henning and Y. Li (2003)
Journal of Cardiovascular Pharmacology and Therapeutics 8, 149-160
   Abstract »    PDF »
Transcriptional regulation of the human NaPi-IIb cotransporter by EGF in Caco-2 cells involves c-myb.
H. Xu, M. Inouye, E. R. Hines, J. F. Collins, and F. K. Ghishan (2003)
Am J Physiol Cell Physiol 284, C1262-C1271
   Abstract »    Full Text »    PDF »
Role of Specific Protein Kinase C Isozymes in Mediating Epidermal Growth Factor, Thyrotropin-Releasing Hormone, and Phorbol Ester Regulation of the Rat Prolactin Promoter in GH4/GH4C1 Pituitary Cells.
C. A. Pickett, N. Manning, Y. Akita, and A. Gutierrez-Hartmann (2002)
Mol. Endocrinol. 16, 2840-2852
   Abstract »    Full Text »    PDF »
Regulation of the PRL Promoter by Akt through cAMP Response Element Binding Protein.
J. Hayakawa, M. Ohmichi, K. Tasaka, Y. Kanda, K. Adachi, Y. Nishio, K. Hisamoto, S. Mabuchi, S. Hinuma, and Y. Murata (2002)
Endocrinology 143, 13-22
   Abstract »    Full Text »    PDF »
Epidermal growth factor regulation of rat NHE2 gene expression.
H. Xu, J. F. Collins, L. Bai, P. R. Kiela, R. M. Lynch, and F. K. Ghishan (2001)
Am J Physiol Cell Physiol 281, C504-C513
   Abstract »    Full Text »    PDF »
Regulation of the human sodium-phosphate cotransporter NaPi-IIb gene promoter by epidermal growth factor.
H. Xu, J. F. Collins, L. Bai, P. R. Kiela, and F. K. Ghishan (2001)
Am J Physiol Cell Physiol 280, C628-C636
   Abstract »    Full Text »    PDF »
Regulation of Transforming Growth Factor alpha  Expression in a Growth Factor-Independent Cell Line.
G. M. Howell, L. E. Humphrey, B. L. Ziober, R. Awwad, B. Periyasamy, A. Koterba, W. Li, J. K. V. Willson, K. Coleman, J. Carboni, et al. (1998)
Mol. Cell. Biol. 18, 303-313
   Abstract »    Full Text »
Stimulation of the Preprothyrotropin-Releasing Hormone Gene by Epidermal Growth Factor.
Y. Ren, T. Satoh, M. Yamada, K. Hashimoto, S. Konaka, T. Iwasaki, and M. Mori (1998)
Endocrinology 139, 195-203
   Abstract »    Full Text »    PDF »
Epidermal Growth Factor Induces CD44 Gene Expression through a Novel Regulatory Element in Mouse Fibroblasts.
M. Zhang, M. H. Wang, R. K. Singh, A. Wells, and G. P. Siegal (1997)
J. Biol. Chem. 272, 14139-14146
   Abstract »    Full Text »    PDF »
GHF-1/Pit-1 Functions as a Cell-specific Integrator of Ras Signaling by Targeting the Ras Pathway to a Composite Ets-1/GHF-1 Response Element.
A. P. Bradford, K. E. Conrad, P. H. Tran, M. C. Ostrowski, and A. Gutierrez-Hartmann (1996)
J. Biol. Chem. 271, 24639-24648
   Abstract »    Full Text »    PDF »
A Composite Ets/Pit-1 Binding Site in the Prolactin Gene Can Mediate Transcriptional Responses to Multiple Signal Transduction Pathways.
P. W. Howard and R. A. Maurer (1995)
J. Biol. Chem. 270, 20930-20936
   Abstract »    Full Text »    PDF »
Epidermal Growth Factor Induces H[IMAGE],K[IMAGE]-ATPase alpha-Subunit Gene Expression through an Element Homologous to the 3` Half-site of the c-fos Serum Response Element.
M. Kaise, A. Muraoka, J. Yamada, and T. Yamada (1995)
J. Biol. Chem. 270, 18637-18642
   Abstract »    Full Text »    PDF »
Epidermal Growth Factor Stimulation of the Human Gastrin Promoter Requires Sp1.
J. L. Merchant, A. Shiotani, E. R. Mortensen, D. K. Shumaker, and D. R. Abraczinskas (1995)
J. Biol. Chem. 270, 6314-6319
   Abstract »    Full Text »    PDF »
Interaction between transcription regulatory regions of prolactin chromatin.
K. Cullen, M. Kladde, and M. Seyfred (1993)
Science 261, 203-206
   Abstract »    PDF »
TEF, a transcription factor expressed specifically in the anterior pituitary during embryogenesis, defines a new class of leucine zipper proteins..
D W Drolet, K M Scully, D M Simmons, M Wegner, K T Chu, L W Swanson, and M G Rosenfeld (1991)
Genes & Dev. 5, 1739-1753
   Abstract »    PDF »
Variable effects of phosphorylation of Pit-1 dictated by the DNA response elements.
M. Kapiloff, Y Farkash, M Wegner, and M. Rosenfeld (1991)
Science 253, 786-789
   Abstract »    PDF »
Pituitary cell phenotypes involve cell-specific Pit-1 mRNA translation and synergistic interactions with other classes of transcription factors..
D M Simmons, J W Voss, H A Ingraham, J M Holloway, R S Broide, M G Rosenfeld, and L W Swanson (1990)
Genes & Dev. 4, 695-711
   Abstract »    PDF »
Mutation of the core or adjacent LVb elements of the Moloney murine leukemia virus enhancer alters disease specificity..
N A Speck, B Renjifo, E Golemis, T N Fredrickson, J W Hartley, and N Hopkins (1990)
Genes & Dev. 4, 233-242
   Abstract »    PDF »
A two-base change in a POU factor-binding site switches pituitary-specific to lymphoid-specific gene expression..
H P Elsholtz, V R Albert, M N Treacy, and M G Rosenfeld (1990)
Genes & Dev. 4, 43-51
   Abstract »    PDF »
Cell-specific expression of the prolactin gene in transgenic mice is controlled by synergistic interactions between promoter and enhancer elements..
E B Crenshaw, K Kalla, D M Simmons, L W Swanson, and M G Rosenfeld (1989)
Genes & Dev. 3, 959-972
   Abstract »    PDF »
Grafting genetically modified cells to the damaged brain: restorative effects of NGF expression.
M. Rosenberg, T Friedmann, R. Robertson, M Tuszynski, J. Wolff, X. Breakefield, and F. Gage (1988)
Science 242, 1575-1578
   Abstract »    PDF »
The c-fos serum response element responds to protein kinase C-dependent and -independent signals but not to cyclic AMP..
M Z Gilman (1988)
Genes & Dev. 2, 394-402
   Abstract »    PDF »
Activation of cell-specific expression of rat growth hormone and prolactin genes by a common transcription factor.
C Nelson, V. Albert, H. Elsholtz, L. Lu, and M. Rosenfeld (1988)
Science 239, 1400-1405
   Abstract »    PDF »
Response and Binding Elements for Ligand-dependent Positive Transcription Factors Integrate Positive and Negative Regulation of Gene Expression.
M.G. Rosenfeld, C.K. Glass, S. Adler, E.B. Crenshaw III, X. He, S.A. Lira, H.P. Elsholtz, H.J. Mangalam, J.M. Holloway, C. Nelson, et al. (1988)
Cold Spring Harb Symp Quant Biol 53, 545-556
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)