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Science 12 December 1986:
Vol. 234. no. 4782, pp. 1417 - 1419
DOI: 10.1126/science.3491427
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Articles

Science, Vol 234, Issue 4782, 1417-1419
Copyright © 1986 by American Association for the Advancement of Science

articles

The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose

LC Sambucetti and T Curran

The properties of the viral and cellular fos proteins (Fos) were investigated as a first step toward understanding the function of the fos gene. Treatment of nuclei with salt and nonionic detergents solubilized a complex that contained Fos together with several other cellular proteins. The majority of the Fos protein complex was released from isolated nuclei incubated in the presence of deoxyribonuclease I or micrococcal nuclease but not with ribonuclease A, suggesting that Fos is associated with chromatin. This hypothesis is supported by the finding that Fos protein from native or denatured nuclear extracts exhibited DNA-binding activity in vitro. These results suggest that Fos is involved in the regulation of gene expression.


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Suppression of c-fos induction in rat brain impairs retention of a brightness discrimination reaction..
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Characterization of a Leucine Zipper-containing Protein Identified by Retroviral Insertion in Avian Neuroretina Cells.
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NeuN, a neuronal specific nuclear protein in vertebrates.
R. Mullen, C. Buck, and A. Smith (1992)
Development 116, 201-211
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Light pulses that shift rhythms induce gene expression in the suprachiasmatic nucleus.
B Rusak, H. Robertson, W Wisden, and S. Hunt (1990)
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Regulation of Proto-Oncogenes and Salivary Gland Cell Proliferation.
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The product of a fos-related gene, fra-1, binds cooperatively to the AP-1 site with Jun: transcription factor AP-1 is comprised of multiple protein complexes..
D R Cohen, P C Ferreira, R Gentz, B R Franza, and T Curran (1989)
Genes & Dev. 3, 173-184
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Fos and Jun bind cooperatively to the AP-1 site: reconstitution in vitro..
F J Rauscher, P J Voulalas, B R Franza, and T Curran (1988)
Genes & Dev. 2, 1687-1699
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Expression of c-fos protein in brain: metabolic mapping at the cellular level.
S. Sagar, F. Sharp, and T Curran (1988)
Science 240, 1328-1331
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Fos-associated protein p39 is the product of the jun proto-oncogene.
F. Rauscher 3rd, D. Cohen, T Curran, T. Bos, P. Vogt, D Bohmann, R Tjian, and B. Franza Jr (1988)
Science 240, 1010-1016
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Constitutive expression of c-fos antisense RNA blocks c-fos gene induction by interferon and by phorbol ester and reduces c-myc expression in F9 embryonal carcinoma cells..
B Z Levi and K Ozato (1988)
Genes & Dev. 2, 554-566
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The Fos complex and Fos-related antigens recognize sequence elements that contain AP-1 binding sites.
B. Franza Jr, F. Rauscher 3rd, S. Josephs, and T Curran (1988)
Science 239, 1150-1153
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Regulation of Proto-oncogene fos: A Paradigm for Early Response Genes.
P. Sassone-Corsi, W.W. Lamph, and I.M. Verma (1988)
Cold Spring Harb Symp Quant Biol 53, 749-760
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Nuclear Oncoproteins Determine the Genetic Program in Response to External Stimuli.
A. Schonthal, S. Gebel, B. Stein, H. Ponta, H.J. Rahmsdorf, and P. Herrlich (1988)
Cold Spring Harb Symp Quant Biol 53, 779-787
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A nerve growth factor-induced gene encodes a possible transcriptional regulatory factor.
J Milbrandt (1987)
Science 238, 797-799
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Mapping patterns of c-fos expression in the central nervous system after seizure.
J. Morgan, D. Cohen, J. Hempstead, and T Curran (1987)
Science 237, 192-197
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