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Science 12 December 1986:
Vol. 234. no. 4782, pp. 1417 - 1419
DOI: 10.1126/science.3491427
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Articles

Science, Vol 234, Issue 4782, 1417-1419
Copyright © 1986 by American Association for the Advancement of Science

articles

The Fos protein complex is associated with DNA in isolated nuclei and binds to DNA cellulose

LC Sambucetti and T Curran

The properties of the viral and cellular fos proteins (Fos) were investigated as a first step toward understanding the function of the fos gene. Treatment of nuclei with salt and nonionic detergents solubilized a complex that contained Fos together with several other cellular proteins. The majority of the Fos protein complex was released from isolated nuclei incubated in the presence of deoxyribonuclease I or micrococcal nuclease but not with ribonuclease A, suggesting that Fos is associated with chromatin. This hypothesis is supported by the finding that Fos protein from native or denatured nuclear extracts exhibited DNA-binding activity in vitro. These results suggest that Fos is involved in the regulation of gene expression.


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