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Science 12 December 1986:
Vol. 234. no. 4782, pp. 1405 - 1408
DOI: 10.1126/science.3491426
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Articles

Science, Vol 234, Issue 4782, 1405-1408
Copyright © 1986 by American Association for the Advancement of Science

articles

Determination of junction avidity of cytolytic T cell and target cell

KL Sung, LA Sung, M Crimmins, SJ Burakoff, and S Chien

A direct measurement of the avidity of the junction between a cytotoxic T lymphocyte and its target cell was achieved by using a biophysical approach. A micromanipulation technique was used to determine the force required to separate a cytotoxic T cell (human clone F1, with specificity for HLA-DRw6) from its specific target cell (JY: HLA-A2, -B7, -DR4, w6) prior to delivery of the lethal hit. The force required to separate the F1-JY pair is 1.5 X 10(4) dynes per square centimeter. This junction avidity for F1-JY pairs is 6 to 13 times greater than that for F1-F1 and JY-JY pairs; the F1-JY conjugate requires a stronger separating force and is more easily rejoined than the homologous cell pairs. This study provides an estimate of the avidity of cytotoxic T cells for their target cells and insights into the biophysical correlates of the molecular complexes formed in the interaction of cytotoxic T cells and their targets during the cytotoxic process.


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