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Science 12 December 1986:
Vol. 234. no. 4782, pp. 1372 - 1378
DOI: 10.1126/science.3097822
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Articles

Science, Vol 234, Issue 4782, 1372-1378
Copyright © 1986 by American Association for the Advancement of Science

articles

The hypogonadal mouse: reproductive functions restored by gene therapy

AJ Mason, SL Pitts, K Nikolics, E Szonyi, JN Wilcox, PH Seeburg, and TA Stewart

The hypogonadal (hpg) mouse lacks a complete gonadotropin-releasing hormone (GnRH) gene and consequently cannot reproduce. Introduction of an intact GnRH gene into the genome of these mutant mice resulted in complete reversal of the hypogonadal phenotype. Transgenic hpg/hpg homozygotes of both sexes were capable of mating and producing offspring. Pituitary and serum concentrations of luteinizing hormone, follicle-stimulating hormone, and prolactin were restored to those of normal animals. Immunocytochemistry and in situ hybridization showed that GnRH expression was restored in the appropriate hypothalamic neurons of the transgenic hpg animals, an indication of neural-specific expression of the introduced gene.


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