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Science 28 November 1986:
Vol. 234. no. 4780, pp. 1099 - 1104
DOI: 10.1126/science.2430332
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Articles

Science, Vol 234, Issue 4780, 1099-1104
Copyright © 1986 by American Association for the Advancement of Science

articles

Efficient trans-splicing of a yeast mitochondrial RNA group II intron implicates a strong 5' exon-intron interaction

A Jacquier and M Rosbash

The reaction mechanism for self-splicing introns requires the existence of a 5' exon binding site on the intron. Experimental evidence is now presented consistent with the existence of such a binding site by demonstrating efficient and accurate trans-self-splicing of a yeast mitochondrial group II intron. Partial and complete trans-splicing reactions take place in the absence of branch formation, part of the usual pathway of nuclear splicing and group II self-splicing. In addition to indicating the existence of a 5' exon binding site on the intron, the results have mechanistic implications for group II self-splicing and perhaps for nuclear splicing as well.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Another Surprise from the Mitochondrial Genome.
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The Therapeutic Potential of Ribozymes.
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Ribozymes: a distinct class of metalloenzymes.
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Science 261, 709-714
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Activation of a cryptic 5' splice site in the upstream exon of the phage T4 td transcript: exon context, missplicing, and mRNA deletion in a fidelity mutant..
P S Chandry and M Belfort (1987)
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The chemistry of self-splicing RNA and RNA enzymes.
T. Cech (1987)
Science 236, 1532-1539
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Long-range Intron-Exon and Intron-Intron Pairings Involved in Self-splicing of Class II Catalytic Introns.
F. Michel and A. Jacquier (1987)
Cold Spring Harb Symp Quant Biol 52, 201-212
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Reactions Mediated by Yeast Mitochondrial Group I and II Introns.
H.F. Tabak, G. Van der Horst, A.J. Winter, J. Smit, R. Van der Veen, J.H.J.M. Kwakman, L.A. Grivell, and A.C. Arnberg (1987)
Cold Spring Harb Symp Quant Biol 52, 213-221
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Group II Intron Self-splicing: Development of Alternative Reaction Conditions and Identification of a Predicted Intermediate.
C.L. Peebles, E.J. Benatan, K.A. Jarrell, and P.S. Perlman (1987)
Cold Spring Harb Symp Quant Biol 52, 223-232
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Characterization of Two RNA-catalyzed RNA Cleavage Reactions.
J.R. Sampson, F.X. Sullivan, L.S. Behlen, A.B. DiRenzo, and O.C. Uhlenbeck (1987)
Cold Spring Harb Symp Quant Biol 52, 267-275
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Genetic System of Chloroplasts.
H. Ozeki, K. Ohyama, H. Inokuchi, H. Fukuzawa, T. Kohchi, T. Sano, K. Nakahigashi, and K. Umesono (1987)
Cold Spring Harb Symp Quant Biol 52, 791-804
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