Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 7 November 1986:
Vol. 234. no. 4777, pp. 746 - 748
DOI: 10.1126/science.3022376
Prev | Table of Contents | Next

Articles

Science, Vol 234, Issue 4777, 746-748
Copyright © 1986 by American Association for the Advancement of Science

articles

Two avirulent herpes simplex viruses generate lethal recombinants in vivo

RT Javier, F Sedarati, and JG Stevens

While it is widely appreciated that infection with a virulent virus can produce disease in an animal, the ability of a mixture of avirulent viruses to produce disease by means of complementation or recombination in vivo has not been established. In this study, two weakly neuroinvasive herpes simplex virus type 1 (HSV-1) strains were simultaneously inoculated onto the footpads of mice. Many (62%) of the animals that received a 1:1 mixture of the viruses died, whereas the animals that received a similar or 100-fold higher dose of each agent alone survived. Of fourteen viruses isolated from the brains of ten mice that died after receiving the mixture of the two weakly neuroinvasive viruses, eleven were recombinants; three of these recombinants were lethal when reapplied to the footpads of mice. These results show that two avirulent HSV-1 variants may interact in vivo to produce virulent recombinants and a lethal infection. They also suggest that different genetic lesions account for the weakly neuroinvasive character of the HSV-1 strains ANG and KOS after footpad inoculation.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Coinfection with Two Closely Related Alphaherpesviruses Results in a Highly Diversified Recombination Mosaic Displaying Negative Genetic Interference.
B. Muylkens, F. Farnir, F. Meurens, F. Schynts, A. Vanderplasschen, M. Georges, and E. Thiry (2009)
J. Virol. 83, 3127-3137
   Abstract »    Full Text »    PDF »
Divergence and Recombination of Clinical Herpes Simplex Virus Type 2 Isolates.
P. Norberg, M. J. Kasubi, L. Haarr, T. Bergstrom, and J.-A. Liljeqvist (2007)
J. Virol. 81, 13158-13167
   Abstract »    Full Text »    PDF »
Competition and complementation between thymidine kinase-negative and wild-type herpes simplex virus during co-infection of mouse trigeminal ganglia.
S.-H. Chen, Y.-W. Lin, A. Griffiths, W.-Y. Huang, and S.-H. Chen (2006)
J. Gen. Virol. 87, 3495-3502
   Abstract »    Full Text »    PDF »
A Full-Genome Phylogenetic Analysis of Varicella-Zoster Virus Reveals a Novel Origin of Replication-Based Genotyping Scheme and Evidence of Recombination between Major Circulating Clades.
G. A. Peters, S. D. Tyler, C. Grose, A. Severini, M. J. Gray, C. Upton, and G. A. Tipples (2006)
J. Virol. 80, 9850-9860
   Abstract »    Full Text »    PDF »
Frequent Coinfection of Cells Explains Functional In Vivo Complementation between Cytomegalovirus Variants in the Multiply Infected Host.
L. Cicin-Sain, J. Podlech, M. Messerle, M. J. Reddehase, and U. H. Koszinowski (2005)
J. Virol. 79, 9492-9502
   Abstract »    Full Text »    PDF »
Interspecific Recombination between Two Ruminant Alphaherpesviruses, Bovine Herpesviruses 1 and 5.
F. Meurens, G. M. Keil, B. Muylkens, S. Gogev, F. Schynts, S. Negro, L. Wiggers, and E. Thiry (2004)
J. Virol. 78, 9828-9836
   Abstract »    Full Text »    PDF »
Superinfection Prevents Recombination of the Alphaherpesvirus Bovine Herpesvirus 1.
F. Meurens, F. Schynts, G. M. Keil, B. Muylkens, A. Vanderplasschen, P. Gallego, and E. Thiry (2004)
J. Virol. 78, 3872-3879
   Abstract »    Full Text »    PDF »
Endocrine Aspects of Cancer Gene Therapy.
L. Barzon, M. Boscaro, and G. Palu (2004)
Endocr. Rev. 25, 1-44
   Abstract »    Full Text »    PDF »
Rise and Survival of Bovine Herpesvirus 1 Recombinants after Primary Infection and Reactivation from Latency.
F. Schynts, F. Meurens, B. Detry, A. Vanderplasschen, and E. Thiry (2003)
J. Virol. 77, 12535-12542
   Abstract »    Full Text »    PDF »
Tyrosine 116 of the Herpes Simplex Virus Type 1 IE{alpha}22 Protein Is an Ocular Virulence Determinant and Potential Phosphorylation Site.
C. R. Brandt and A. W. Kolb (2003)
Invest. Ophthalmol. Vis. Sci. 44, 4601-4607
   Abstract »    Full Text »    PDF »
Multiple Determinants Contribute to the Virulence of HSV Ocular and CNS Infection and Identification of Serine 34 of the US1 Gene as an Ocular Disease Determinant.
C. R. Brandt, A. W. Kolb, D. D. Shah, A. M. Pumfery, R. L. Kintner, E. Jaehnig, and J. J. Van Gompel (2003)
Invest. Ophthalmol. Vis. Sci. 44, 2657-2668
   Abstract »    Full Text »    PDF »
Attenuated, Replication-Competent Herpes Simplex Virus Type 1 Mutant G207: Safety Evaluation in Mice.
P. Sundaresan, W. D. Hunter, R. L. Martuza, and S. D. Rabkin (2000)
J. Virol. 74, 3832-3841
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)