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Science 7 November 1986:
Vol. 234. no. 4777, pp. 728 - 731
DOI: 10.1126/science.3490689
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Articles

Science, Vol 234, Issue 4777, 728-731
Copyright © 1986 by American Association for the Advancement of Science

articles

T-lymphocyte priming and protection against Friend leukemia by vaccinia-retrovirus env gene recombinant

PL Earl, B Moss, RP Morrison, K Wehrly, J Nishio, and B Chesebro

The current prevalence of the acquired immune deficiency syndrome in humans has provoked renewed interest in methods of protective immunization against retrovirus-induced diseases. In this study, a vaccinia-retrovirus recombinant vector was constructed to study mechanisms of immune protection against Friend virus leukemia in mice. The envelope (env) gene from Friend murine leukemia virus (F-MuLV) was inserted into the genome of a vaccinia virus expression vector. Infected cells synthesized gp85, the glycosylated primary product of the env gene. Processing to gp70 and p15E, and cell surface localization, were similar to that occurring in cells infected with F-MuLV. Mice inoculated with live recombinant vaccinia virus had an envelope-specific T-cell proliferative response and, after challenge with Friend virus complex, developed neutralizing antibody and cytotoxic T cells (CTL) and were protected against leukemia. In contrast, unimmunized and control groups developed a delayed neutralizing antibody response, but no detectable CTL, and succumbed to leukemia. Genes of the major histocompatibility complex influenced protection induced by the vaccinia recombinant but not that induced by attenuated N-tropic Friend virus.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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Critical Role for CD4+ T Cells in Controlling Retrovirus Replication and Spread in Persistently Infected Mice.
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J. Virol. 72, 6559-6564
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Immunity to retroviral infection: The Friend virus model.
K. J. Hasenkrug and B. Chesebro (1997)
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Vaccinia virus: a tool for research and vaccine development.
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Recombinant virus vaccine-induced SIV-specific CD8+ cytotoxic T lymphocytes.
L Shen, Z. Chen, M. Miller, V Stallard, G. Mazzara, D. Panicali, and N. Letvin (1991)
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The role of B cells for in vivo T cell responses to a Friend virus-induced leukemia.
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Development and Evaluation of a Vaccine for Human Immunodeficiency Virus (HIV) Infection.
A. S. Fauci, R. C. Gallo, S. Koenig, J. Salk, and R. H. Purcell (1989)
Ann Intern Med 110, 373-385
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Reactivity and Tolerance of Virus-specific T Cells.
R.M. Zinkernagel, H.P. Pircher, M. Schulz, T. Leist, S. Oehen, and H. Hengartner (1989)
Cold Spring Harb Symp Quant Biol 54, 843-851
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Immunology of Human Immunodeficiency Virus Infection and the Acquired Immunodeficiency Syndrome: An Update.
M. SELIGMANN, A. J. PINCHING, F. S. ROSEN, J. L. FAHEY, R. M. KHAITOV, D. KLATZMANN, S. KOENIG, N. LUO, J. NGU, G. RIETHMULLER, et al. (1987)
Ann Intern Med 107, 234-242
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