Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 3 October 1986:
Vol. 234. no. 4772, pp. 53 - 59
DOI: 10.1126/science.3018926
Prev | Table of Contents | Next

Articles

Science, Vol 234, Issue 4772, 53-59
Copyright © 1986 by American Association for the Advancement of Science

articles

A genetic approach to promoter recognition during trans induction of viral gene expression

DM Coen, SP Weinheimer, and SL McKnight

Viral infection of mammalian cells entails the regulated induction of viral gene expression. The induction of many viral genes, including the herpes simplex virus gene encoding thymidine kinase (tk), depends on viral regulatory proteins that act in trans. Because recognition of the tk promoter by cellular transcription factors is well understood, its trans induction by viral regulatory proteins may serve as a useful model for the regulation of eukaryotic gene expression. A comprehensive set of mutations was therefore introduced into the chromosome of herpes simplex virus at the tk promoter to directly analyze the effects of promoter mutations on tk transcription. The promoter domains required for efficient tk expression under conditions of trans induction corresponded to those important for recognition by cellular transcription factors. Thus, trans induction of tk expression may be catalyzed initially by the interaction of viral regulatory proteins with cellular transcription factors.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Stabilized Binding of TBP to the TATA Box of Herpes Simplex Virus Type 1 Early (tk) and Late (gC) Promoters by TFIIA and ICP4.
S. E. Zabierowski and N. A. DeLuca (2008)
J. Virol. 82, 3546-3554
   Abstract »    Full Text »    PDF »
Binding of ICP4, TATA-Binding Protein, and RNA Polymerase II to Herpes Simplex Virus Type 1 Immediate-Early, Early, and Late Promoters in Virus-Infected Cells.
P. Sampath and N. A. DeLuca (2008)
J. Virol. 82, 2339-2349
   Abstract »    Full Text »    PDF »
Expression of Extremely Low Levels of Thymidine Kinase from an Acyclovir-Resistant Herpes Simplex Virus Mutant Supports Reactivation from Latently Infected Mouse Trigeminal Ganglia.
M. I. Besecker, C. L. Furness, D. M. Coen, and A. Griffiths (2007)
J. Virol. 81, 8356-8360
   Abstract »    Full Text »    PDF »
Low-Level Expression and Reversion both Contribute to Reactivation of Herpes Simplex Virus Drug-Resistant Mutants with Mutations on Homopolymeric Sequences in Thymidine Kinase..
A. Griffiths, M. A. Link, C. L. Furness, and D. M. Coen (2006)
J. Virol. 80, 6568-6574
   Abstract »    Full Text »    PDF »
An unusual internal ribosome entry site in the herpes simplex virus thymidine kinase gene.
A. Griffiths and D. M. Coen (2005)
PNAS 102, 9667-9672
   Abstract »    Full Text »    PDF »
High-Frequency Phenotypic Reversion and Pathogenicity of an Acyclovir-Resistant Herpes Simplex Virus Mutant.
A. Griffiths and D. M. Coen (2003)
J. Virol. 77, 2282-2286
   Abstract »    Full Text »    PDF »
Phosphorylation of Transcription Factor Sp1 during Herpes Simplex Virus Type 1 Infection.
D.-B. Kim and N. A. DeLuca (2002)
J. Virol. 76, 6473-6479
   Abstract »    Full Text »    PDF »
The Initiator Element in a Herpes Simplex Virus Type 1 Late-Gene Promoter Enhances Activation by ICP4, Resulting in Abundant Late-Gene Expression.
D.-B. Kim, S. Zabierowski, and N. A. DeLuca (2002)
J. Virol. 76, 1548-1558
   Abstract »    Full Text »    PDF »
Repression of viral transcription during herpes simplex virus latency.
C. M. Preston (2000)
J. Gen. Virol. 81, 1-19
   Full Text »
The Role of Herpes Simplex Virus ICP27 in the Regulation of UL24 Gene Expression by Differential Polyadenylation.
L. E. Hann, W. J. Cook, S. L. Uprichard, D. M. Knipe, and D. M. Coen (1998)
J. Virol. 72, 7709-7714
   Abstract »    Full Text »    PDF »
Human Thymidine Kinase Can Functionally Replace Herpes Simplex Virus Type 1 Thymidine Kinase for Viral Replication in Mouse Sensory Ganglia and Reactivation from Latency upon Explant.
S.-H. Chen, W. J. Cook, K. L. Grove, and D. M. Coen (1998)
J. Virol. 72, 6710-6715
   Abstract »    Full Text »    PDF »
The High Mobility Group Protein 1 Is a Coactivator of Herpes Simplex Virus ICP4 In Vitro.
M. J. Carrozza and N. DeLuca (1998)
J. Virol. 72, 6752-6757
   Abstract »    Full Text »    PDF »
Temporal Gene Regulation of the Channel Catfish Virus (Ictalurid Herpesvirus 1).
S. Huang and L. A. Hanson (1998)
J. Virol. 72, 1910-1917
   Abstract »    Full Text »    PDF »
The pseudorabies immediate early protein stimulates in vitro transcription by facilitating TFIID: promoter interactions..
S M Abmayr, J L Workman, and R G Roeder (1988)
Genes & Dev. 2, 542-553
   Abstract »    PDF »
Constraints on spacing between transcription factor binding sites in a simple adenovirus promoter..
L Wu and A Berk (1988)
Genes & Dev. 2, 403-411
   Abstract »    PDF »
A specific 15-bp TATA box promoter element is required for expression of a herpes simplex virus type 1 late gene..
F L Homa, J C Glorioso, and M Levine (1988)
Genes & Dev. 2, 40-53
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)