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Science 26 September 1986:
Vol. 233. no. 4771, pp. 1403 - 1408
DOI: 10.1126/science.3529391
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Articles

Science, Vol 233, Issue 4771, 1403-1408
Copyright © 1986 by American Association for the Advancement of Science

articles

A genetic approach to analyzing membrane protein topology

C Manoil and J Beckwith

Fusions of the secreted protein alkaline phosphatase to an integral cytoplasmic membrane protein of Escherichia coli showed different activities depending on where in the membrane protein the alkaline phosphatase was fused. Fusions to positions in or near the periplasmic domain led to high alkaline phosphatase activity, whereas those to positions in the cytoplasmic domain gave low activity. Analysis of alkaline phosphatase fusions to membrane proteins of unknown structure may thus be generally useful in determining their membrane topologies.


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Transmembrane Topology of alpha - and beta -Subunits of Na+,K+-ATPase Derived from beta -Galactosidase Fusion Proteins Expressed in Yeast.
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Membrane Topology of the Sodium Ion-dependent Citrate Carrier of Klebsiella pneumoniae. EVIDENCE FOR A NEW STRUCTURAL CLASS OF SECONDARY TRANSPORTERS.
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Determination of the Transmembrane Topology of Yeast Sec61p, an Essential Component of the Endoplasmic Reticulum Translocation Complex.
B. M. Wilkinson, A. J. Critchley, and C. J. Stirling (1996)
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Human Protein Disulfide Isomerase Functionally Complements a dsbA Mutation and Enhances the Yield of Pectate Lyase C in Escherichia coli.
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Harpin, elicitor of the hypersensitive response produced by the plant pathogen Erwinia amylovora.
Z. Wei, R. Laby, C. Zumoff, D. Bauer, S. He, A Collmer, and S. Beer (1992)
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The secE gene encodes an integral membrane protein required for protein export in Escherichia coli..
P J Schatz, P D Riggs, A Jacq, M J Fath, and J Beckwith (1989)
Genes & Dev. 3, 1035-1044
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Bacterial motility: membrane topology of the Escherichia coli MotB protein.
S. Chun and J. Parkinson (1988)
Science 239, 276-278
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Structure-Function Studies of Bacterial Chemosensors.
P. Ames, J. Chen, C. Wolff, and J.S. Parkinson (1988)
Cold Spring Harb Symp Quant Biol 53, 59-65
   Abstract »    PDF »
Global flexibility in a sensory receptor: a site-directed cross-linking approach.
J. Falke and D. Koshland Jr (1987)
Science 237, 1596-1600
   Abstract »    PDF »
A Method for Determining the in Vivo Topology of Yeast Polytopic Membrane Proteins Demonstrates That Gap1p Fully Integrates into the Membrane Independently of Shr3p.
C. F. Gilstring and P. O. Ljungdahl (2000)
J. Biol. Chem. 275, 31488-31495
   Abstract »    Full Text »    PDF »
The Internal Repeats in the Na+/Ca2+ Exchanger-related Escherichia coli Protein YrbG Have Opposite Membrane Topologies.
A. Saaf, L. Baars, and G. von Heijne (2001)
J. Biol. Chem. 276, 18905-18907
   Abstract »    Full Text »    PDF »
Pectate lyase A, an enzymatic subunit of the Clostridium cellulovorans cellulosome.
Y. Tamaru and R. H. Doi (2001)
PNAS 98, 4125-4129
   Abstract »    Full Text »    PDF »
YgbQ, a cell division protein in Escherichia coli and Vibrio cholerae, localizes in codependent fashion with FtsL to the division site.
N. Buddelmeijer, N. Judson, D. Boyd, J. J. Mekalanos, and J. Beckwith (2002)
PNAS 99, 6316-6321
   Abstract »    Full Text »    PDF »
Evidence in support of a four transmembrane-pore-transmembrane topology model for the Arabidopsis thaliana Na+/K+ translocating AtHKT1 protein, a member of the superfamily of K+ transporters.
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   Abstract »    Full Text »    PDF »


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