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Science 12 September 1986:
Vol. 233. no. 4769, pp. 1175 - 1180
DOI: 10.1126/science.3526554

Articles

Science, Vol 233, Issue 4769, 1175-1180
Copyright © 1986 by American Association for the Advancement of Science


articles

A yeast gene that is essential for release from glucose repression encodes a protein kinase

JL Celenza and M Carlson

The SNF1 gene plays a central role in carbon catabolite repression in the yeast Saccharomyces cerevisiae, namely that SNF1 function is required for expression of glucose-repressible genes. The nucleotide sequence of the cloned SNF1 gene was determined, and the predicted amino acid sequence shows that SNF1 encodes a 72,040-dalton polypeptide that has significant homology to the conserved catalytic domain of mammalian protein kinases. Specific antisera were prepared and used to identify the SNF1 protein. The protein was shown to transfer phosphate from adenosine triphosphate to serine and threonine residues in an in vitro autophosphorylation reaction. These findings indicate that SNF1 encodes a protein kinase and suggest that protein phosphorylation plays a critical role in regulation by carbon catabolite repression in eukaryotic cells.


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B. J. Michell, D. Stapleton, K. I. Mitchelhill, C. M. House, F. Katsis, L. A. Witters, and B. E. Kemp (1996)
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Characterization of a unique protein component of yeast RNase MRP: an RNA-binding protein with a zinc-cluster domain..
M E Schmitt and D A Clayton (1994)
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Caenorhabditis elegans unc-51 gene required for axonal elongation encodes a novel serine/threonine kinase..
K Ogura, C Wicky, L Magnenat, H Tobler, I Mori, F Muller, and Y Ohshima (1994)
Genes & Dev. 8, 2389-2400
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Yeast SNF2/SWI2, SNF5, and SNF6 proteins function coordinately with the gene-specific transcriptional activators GAL4 and Bicoid..
B C Laurent and M Carlson (1992)
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X Yang, E. Hubbard, and M Carlson (1992)
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S. Hanks, A. Quinn, and T Hunter (1988)
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In situ detection of sequence-specific DNA binding activity specified by a recombinant bacteriophage..
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Genes & Dev. 2, 801-806
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WaaP of Pseudomonas aeruginosa Is a Novel Eukaryotic Type Protein-tyrosine Kinase as Well as a Sugar Kinase Essential for the Biosynthesis of Core Lipopolysaccharide.
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Science. ISSN 0036-8075 (print), 1095-9203 (online)