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Science 22 August 1986:
Vol. 233. no. 4766, pp. 883 - 886
DOI: 10.1126/science.3738514
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Articles

Science, Vol 233, Issue 4766, 883-886
Copyright © 1986 by American Association for the Advancement of Science

articles

Replication of the B19 parvovirus in human bone marrow cell cultures

K Ozawa, G Kurtzman, and N Young

The B19 parvovirus is responsible for at least three human diseases. The virus was successfully propagated in suspension cultures of human erythroid bone marrow from patients with hemolytic anemias; release of newly synthesized virus into the supernatants of infected cultures was observed. This culture system allowed study at a molecular level of events associated with the B19 life cycle. The B19 parvovirus replicated through high molecular weight intermediate forms, linked through a terminal hairpin structure. B19 replication in vitro was highly dependent on the erythropoietic content of cultures and on addition of the hormone erythropoietin.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Block to the Production of Full-Length B19 Virus Transcripts by Internal Polyadenylation Is Overcome by Replication of the Viral Genome.
W. Guan, F. Cheng, Y. Yoto, S. Kleiboeker, S. Wong, N. Zhi, D. J. Pintel, and J. Qiu (2008)
J. Virol. 82, 9951-9963
   Abstract »    Full Text »    PDF »
Human B19 Erythrovirus In Vitro Replication: What's New?.
S. Pillet, S. Fichelson, F. Morinet, N. S. Young, N. Zhi, and S. Wong (2008)
J. Virol. 82, 8951-8953
   Full Text »    PDF »
Ex Vivo-Generated CD36+ Erythroid Progenitors Are Highly Permissive to Human Parvovirus B19 Replication.
S. Wong, N. Zhi, C. Filippone, K. Keyvanfar, S. Kajigaya, K. E. Brown, and N. S. Young (2008)
J. Virol. 82, 2470-2476
   Abstract »    Full Text »    PDF »
Biological and Immunological Relations among Human Parvovirus B19 Genotypes 1 to 3.
A. Ekman, K. Hokynar, L. Kakkola, K. Kantola, L. Hedman, H. Bonden, M. Gessner, C. Aberham, P. Norja, S. Miettinen, et al. (2007)
J. Virol. 81, 6927-6935
   Abstract »    Full Text »    PDF »
Bioportfolio: Lifelong persistence of variant and prototypic erythrovirus DNA genomes in human tissue.
P. Norja, K. Hokynar, L.-M. Aaltonen, R. Chen, A. Ranki, E. K. Partio, O. Kiviluoto, I. Davidkin, T. Leivo, A. M. Eis-Hubinger, et al. (2006)
PNAS 103, 7450-7453
   Abstract »    Full Text »    PDF »
Parvovirus B19-Induced Apoptosis of Hepatocytes.
B. D. Poole, Y. V. Karetnyi, and S. J. Naides (2004)
J. Virol. 78, 7775-7783
   Abstract »    Full Text »    PDF »
Parvovirus B19.
N. S. Young and K. E. Brown (2004)
N. Engl. J. Med. 350, 586-597
   Full Text »    PDF »
{alpha}5{beta}1 integrin as a cellular coreceptor for human parvovirus B19: requirement of functional activation of {beta}1 integrin for viral entry.
K. A. Weigel-Kelley, M. C. Yoder, and A. Srivastava (2003)
Blood 102, 3927-3933
   Abstract »    Full Text »    PDF »
Human Parvovirus B19.
E. D. Heegaard and K. E. Brown (2002)
Clin. Microbiol. Rev. 15, 485-505
   Abstract »    Full Text »    PDF »
A transgenic mouse model for non-immune hydrops fetalis induced by the NS1 gene of human parvovirus B19.
H. Chisaka, E. Morita, K. Murata, N. Ishii, N. Yaegashi, K. Okamura, and K. Sugamura (2002)
J. Gen. Virol. 83, 273-281
   Abstract »    Full Text »    PDF »
Human Parvovirus B19 Induces Cell Cycle Arrest at G2 Phase with Accumulation of Mitotic Cyclins.
E. Morita, K. Tada, H. Chisaka, H. Asao, H. Sato, N. Yaegashi, and K. Sugamura (2001)
J. Virol. 75, 7555-7563
   Abstract »    Full Text »    PDF »
Recombinant Human Parvovirus B19 Vectors: Erythrocyte P Antigen Is Necessary but Not Sufficient for Successful Transduction of Human Hematopoietic Cells.
K. A. Weigel-Kelley, M. C. Yoder, and A. Srivastava (2001)
J. Virol. 75, 4110-4116
   Abstract »    Full Text »
Pathogenesis of human parvovirus B19 in rheumatic disease.
J. R Kerr (2000)
Ann Rheum Dis 59, 672-683
   Full Text »
Integrity and full coding sequence of B19 virus DNA persisting in human synovial tissue.
K. Hokynar, J. Brunstein, M. Söderlund-Venermo, O. Kiviluoto, E. K. Partio, Y. Konttinen, and K. Hedman (2000)
J. Gen. Virol. 81, 1017-1025
   Abstract »    Full Text »
Recombinant Human Parvovirus B19 Vectors: Erythroid Cell-Specific Delivery and Expression of Transduced Genes.
S. Ponnazhagan, K. A. Weigel, S. P. Raikwar, P. Mukherjee, M. C. Yoder, and A. Srivastava (1998)
J. Virol. 72, 5224-5230
   Abstract »    Full Text »    PDF »
Regulation of Human B19 Parvovirus Promoter Expression by hGABP (E4TF1) Transcription Factor.
I. Vassias, U. Hazan, Y. Michel, C. Sawa, H. Handa, L. Gouya, and F. Morinet (1998)
J. Biol. Chem. 273, 8287-8293
   Abstract »    Full Text »    PDF »
Parvoviruses and bone marrow failure.
K. Brown and N. Young (1996)
Stem Cells 14, 151-163
   Abstract »
Resistance to Parvovirus B19 Infection Due to Lack of Virus Receptor (Erythrocyte P Antigen).
K. E. Brown, J. R. Hibbs, G. Gallinella, S. M. Anderson, E. D. Lehman, P. McCarthy, and N. S. Young (1994)
N. Engl. J. Med. 330, 1192-1196
   Abstract »    Full Text »
Use of Gamma Globulin and Erythropoietin in a Sickle Cell Aplastic Crisis.
A. D. Lascari and J. M. Pearce (1994)
Clinical Pediatrics 33, 117-119
   PDF »
Fifth Disease: A Brief Review of Infections in Childhood, in Adulthood, and in Pregnancy.
H. M. Feder Jr and I. Anderson (1989)
Arch Intern Med 149, 2176-2178
   Abstract »    PDF »
Risks Associated With Human Parvovirus B19 Infection.
(1989)
Arch Dermatol 125, 475-480
   Abstract »    PDF »
Risks Associated With Human Parvovirus B19 Infection.
(1989)
JAMA 261, 1555-1563
   PDF »


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