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Science 15 August 1986:
Vol. 233. no. 4765, pp. 778 - 780
DOI: 10.1126/science.3755546
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Articles

Science, Vol 233, Issue 4765, 778-780
Copyright © 1986 by American Association for the Advancement of Science

articles

Amplification of an esterase gene is responsible for insecticide resistance in a California Culex mosquito

C Mouches, N Pasteur, JB Berge, O Hyrien, M Raymond, BR de Saint Vincent, M de Silvestri, and GP Georghiou

An esterase gene from the mosquito Culex quinquefasciatus that is responsible for resistance to a variety of organophosphorus (OP) insecticides was cloned in lambda gt11 phage. This gene was used to investigate the genetic mechanism of the high production of the esterase B1 it encodes in OP-resistant Culex quinquefasciatus Say (Tem-R strain) from California. Adults of the Tem-R strain were found to possess at least 250 times more copies of the gene than adults of a susceptible strain (S-Lab). The finding that selection by pesticides may result in the amplification of genes encoding detoxifying enzymes in whole, normally developed, reproducing insects emphasizes the biological importance of this mechanism and opens new areas of investigation in pesticide resistance management.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
PCR-BASED ASSAY TO SURVEY FOR KNOCKDOWN RESISTANCE TO PYRETHROID ACARICIDES IN HUMAN SCABIES MITES (SARCOPTES SCABIEI VAR HOMINIS).
C. PASAY, S. WALTON, K. FISCHER, D. HOLT, and J. MCCARTHY (2006)
Am J Trop Med Hyg 74, 649-657
   Abstract »    Full Text »    PDF »
Cyp12a4 confers lufenuron resistance in a natural population of Drosophila melanogaster.
M. R. Bogwitz, H. Chung, L. Magoc, S. Rigby, W. Wong, M. O'Keefe, J. A. McKenzie, P. Batterham, and P. J. Daborn (2005)
PNAS 102, 12807-12812
   Abstract »    Full Text »    PDF »
Insecticide resistance resulting from an absence of target-site gene product.
T. G. Wilson and M. Ashok (1998)
PNAS 95, 14040-14044
   Abstract »    Full Text »    PDF »
Proteinase-mediated Insect Resistance to Bacillus thuringiensis Toxins.
B. Oppert, K. J. Kramer, R. W. Beeman, D. Johnson, and W. H. McGaughey (1997)
J. Biol. Chem. 272, 23473-23476
   Abstract »    Full Text »    PDF »
Mosquito Carboxylesterase Est[IMAGE]2[IMAGE] (A[IMAGE]).
A. Vaughan and J. Hemingway (1995)
J. Biol. Chem. 270, 17044-17049
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)