Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 25 July 1986:
Vol. 233. no. 4762, pp. 472 - 475
DOI: 10.1126/science.2425433
Prev | Table of Contents | Next

Articles

Science, Vol 233, Issue 4762, 472-475
Copyright © 1986 by American Association for the Advancement of Science

articles

A poliovirus neutralization epitope expressed on hybrid hepatitis B surface antigen particles

F Delpeyroux, N Chenciner, A Lim, Y Malpiece, B Blondel, R Crainic, S van der Werf, and RE Streeck

The hepatitis B virus (HBV) envelope protein carrying the surface antigen (HBsAg) is assembled with cellular lipids in mammalian cells into empty viral envelopes. In a study to evaluate the capacity of such particles to present foreign peptide sequences in a biologically active form, in-phase insertions were created in the S gene encoding the major envelope protein. One of the sequences inserted was a synthetic DNA fragment encoding a poliovirus neutralization epitope. Mammalian cells expressing the modified gene secreted hybrid particles closely resembling authentic 22-nanometer HBsAg particles. These particles reacted with a poliovirus-specific monoclonal antibody and induced neutralizing antibodies against poliovirus. The results indicate that empty viral envelopes of HBV may provide a means for the presentation of peptide sequences and for their export from mammalian cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Low-Dose Adenovirus Vaccine Encoding Chimeric Hepatitis B Virus Surface Antigen-Human Papillomavirus Type 16 E7 Proteins Induces Enhanced E7-Specific Antibody and Cytotoxic T-Cell Responses.
A. Baez-Astua, E. Herraez-Hernandez, N. Garbi, H. A. Pasolli, V. Juarez, H. zur Hausen, and A. Cid-Arregui (2005)
J. Virol. 79, 12807-12817
   Abstract »    Full Text »    PDF »
Identification of Immunogenic Hot Spots within Plum Pox Potyvirus Capsid Protein for Efficient Antigen Presentation.
M. R. Fernandez-Fernandez, J. L. Martinez-Torrecuadrada, F. Roncal, E. Dominguez, and J. A. Garcia (2002)
J. Virol. 76, 12646-12653
   Abstract »    Full Text »    PDF »
Antigenicity and Immunogenicity of Novel Chimeric Hepatitis B Surface Antigen Particles with Exposed Hepatitis C Virus Epitopes.
H. J. Netter, T. B. Macnaughton, W.-P. Woo, R. Tindle, and E. J. Gowans (2001)
J. Virol. 75, 2130-2141
   Abstract »    Full Text »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)