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Science 27 June 1986:
Vol. 232. no. 4758, pp. 1627 - 1629
DOI: 10.1126/science.3715468
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Articles

Science, Vol 232, Issue 4758, 1627-1629
Copyright © 1986 by American Association for the Advancement of Science

articles

Acetylcholine receptor synthesis in retina and transport to optic tectum in goldfish

JM Henley, JM Lindstrom, and RE Oswald

Previous studies have suggested that the retinotectal system of the goldfish contains a nicotinic acetylcholine receptor (nAChR) that is sensitive to alpha-bungarotoxin. Extracellularly recorded field potentials elicited in response to visual stimulation can be blocked by alpha-bungarotoxin, and alpha-bungarotoxin can interfere with the maintenance of retinotectal synaptic connections. Whether the transmission between the retinal ganglion cells and the tectal cells is mediated by acetylcholine and whether nAChR's exist on the dendrites of tectal cells are questions that remain. The experiments described in this report were designed to determine the site of synthesis of the nAChR's associated with the goldfish retinotectal projection. Radioactive (35S-labeled) methionine was injected into either the eye or the tectal ventricle, and the incorporation of radioactivity into the nAChR was measured by immunoprecipitation. The use of this technique provides evidence that an nAChR associated with the goldfish retinotectal projection is synthesized in the retina and transported to the optic tectum, which suggests a presynaptic site of acetylcholine action on retinal terminals.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Nicotinic Cholinergic Receptors in the Rat Retina: Simple and Mixed Heteromeric Subtypes.
A. M. Marritt, B. C. Cox, R. P. Yasuda, J. M. McIntosh, Y. Xiao, B. B. Wolfe, and K. J. Kellar (2005)
Mol. Pharmacol. 68, 1656-1668
   Abstract »    Full Text »    PDF »
Heterogeneity and Selective Targeting of Neuronal Nicotinic Acetylcholine Receptor (nAChR) Subtypes Expressed on Retinal Afferents of the Superior Colliculus and Lateral Geniculate Nucleus: Identification of a New Native nAChR Subtype {alpha}3{beta}2({alpha}5 or {beta}3) Enriched in Retinocollicular Afferents.
C. Gotti, M. Moretti, A. Zanardi, A. Gaimarri, N. Champtiaux, J.-P. Changeux, P. Whiteaker, M. J. Marks, F. Clementi, and M. Zoli (2005)
Mol. Pharmacol. 68, 1162-1171
   Abstract »    Full Text »    PDF »
Light-Induced Calcium Influx Into Retinal Axons Is Regulated by Presynaptic Nicotinic Acetylcholine Receptor Activity In Vivo.
J. A. Edwards and H. T. Cline (1999)
J Neurophysiol 81, 895-907
   Abstract »    Full Text »    PDF »
Direct Recording of Nicotinic Responses in Presynaptic Nerve Terminals.
J. S. Coggan, J. Paysan, W. G. Conroy, and D. K. Berg (1997)
J. Neurosci. 17, 5798-5806
   Abstract »    Full Text »    PDF »
Role of Ca2+ Ions in Nicotinic Facilitation of GABA Release in Mouse Thalamus.
C. Lena and J.-P. Changeux (1997)
J. Neurosci. 17, 576-585
   Abstract »    Full Text »    PDF »
5HT1B Receptor Agonists Inhibit Light-Induced Phase Shifts of Behavioral Circadian Rhythms and Expression of the Immediate-Early Gene c-fos in the Suprachiasmatic Nucleus.
G. E. Pickard, E. T. Weber, P. A. Scott, A. F. Riberdy, and M. A. Rea (1996)
J. Neurosci. 16, 8208-8220
   Abstract »    Full Text »    PDF »


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Science. ISSN 0036-8075 (print), 1095-9203 (online)