Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 20 June 1986:
Vol. 232. no. 4757, pp. 1543 - 1545
DOI: 10.1126/science.3487116
Prev | Table of Contents | Next

Articles

Science, Vol 232, Issue 4757, 1543-1545
Copyright © 1986 by American Association for the Advancement of Science

articles

Blockade of visual excitation and adaptation in Limulus photoreceptor by GDP-beta-S

A Fein

Light causes both depolarization and adaptation to light in Limulus ventral photoreceptors. Both visual excitation and adaptation were blocked by guanosine 5'-O-(2-thiodiphosphate) (GDP-beta-S), a metabolically stable analog of guanosine 5'-diphosphate (GDP). However, GDP-beta-S did not block the excitation caused by injection of inositol 1,4,5-trisphosphate into the cell. These results suggest a molecular cascade of visual excitation and adaptation: Light isomerizes the visual pigment rhodopsin, which in turn activates a guanyl nucleotide-binding protein. The binding protein then stimulates production of inositol 1,4,5-trisphosphate, which causes release of calcium from the endoplasmic reticulum.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Rapid Coupling of Calcium Release to Depolarization in Limulus polyphemus Ventral Photoreceptors as Revealed by Microphotolysis and Confocal Microscopy.
K. Ukhanov and R. Payne (1997)
J. Neurosci. 17, 1701-1709
   Abstract »    Full Text »    PDF »
G Protein Control of Drosophila Photoreceptor Phospholipase C.
J. L. R. Deer, J. B. Hurley, and S. L. Yarfitz (1995)
J. Biol. Chem. 270, 12623-12628
   Abstract »    Full Text »    PDF »
Purification, Characterization, and Partial Amino Acid Sequence of a G Protein-activated Phospholipase C from Squid Photoreceptors.
J. Mitchell, J. Gutierrez, and J. K. Northup (1995)
J. Biol. Chem. 270, 854-859
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)