Plant glutamine synthetase complements a glnA mutation in Escherichia coli

doi:10.1126/science.2871626

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Science 6 June 1986:
Vol. 232. no. 4755, pp. 1242 - 1244
DOI: 10.1126/science.2871626

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Science, Vol 232, Issue 4755, 1242-1244
Copyright © 1986 by American Association for the Advancement of Science

articles

Plant glutamine synthetase complements a glnA mutation in Escherichia coli

S DasSarma, E Tischer, and HM Goodman

A glutamine synthetase gene from alfalfa (Medicago sativa) has been expressed in Escherichia coli after fusion of bacterial transcription and translation signals to a complete alfalfa glutamine synthetase coding sequence. Synthesis of the alfalfa glutamine synthetase enzyme in Escherichia coli was demonstrated by functional genetic complementation of a glutamine synthetase-deficient mutant and by immunoblotting analysis. These results should facilitate protein engineering and structure-function analysis of the plant enzyme.

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