Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 30 May 1986:
Vol. 232. no. 4754, pp. 1123 - 1127
DOI: 10.1126/science.3010463
Prev | Table of Contents | Next

Articles

Science, Vol 232, Issue 4754, 1123-1127
Copyright © 1986 by American Association for the Advancement of Science

articles

AIDS retrovirus induced cytopathology: giant cell formation and involvement of CD4 antigen

JD Lifson, GR Reyes, MS McGrath, BS Stein, and EG Engleman

The formation of multinucleated giant cells with progression to cell death is a characteristic manifestation of the cytopathology induced by the AIDS retrovirus in infected T lymphoid cells. The mechanism of giant cell formation was studied in the CD4 (T4/Leu 3) positive T cell lines JM (Jurkat) and VB and in variants of these lines that are negative for cell surface CD4 antigen. By means of a two-color fluorescent labeling technique, multinucleated giant cells in infected cultures were shown to form through cell fusion. Antibody to CD4 specifically inhibited fusion, and uninfected CD4 negative cells, in contrast to uninfected CD4 positive cells, did not undergo fusion with infected cells, suggesting a direct role for the CD4 antigen in the process of syncytium formation. These results suggest that, in vivo, cell fusion involving the CD4 molecule may represent a mechanism whereby uninfected cells can be incorporated into AIDS virus infected syncytia. Because the giant cells die soon after they are formed, this process may contribute to the depletion of helper/inducer T cells characteristically observed in AIDS.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Preferential Cytolysis of Peripheral Memory CD4+ T Cells by In Vitro X4-Tropic Human Immunodeficiency Virus Type 1 Infection before the Completion of Reverse Transcription.
Y. Zhou, L. Shen, H.-C. Yang, and R. F. Siliciano (2008)
J. Virol. 82, 9154-9163
   Abstract »    Full Text »    PDF »
Induction of apoptosis by TNF receptor 2 in a T-cell hybridoma is FADD dependent and blocked by caspase-8 inhibitors.
B. Depuydt, G. Van Loo, P. Vandenabeele, and W. Declercq (2005)
J. Cell Sci. 118, 497-504
   Abstract »    Full Text »    PDF »
Host Cell Gene Expression during Human Immunodeficiency Virus Type 1 Latency and Reactivation and Effects of Targeting Genes That Are Differentially Expressed in Viral Latency.
V. Krishnan and S. L. Zeichner (2004)
J. Virol. 78, 9458-9473
   Abstract »    Full Text »    PDF »
Macrophage HIV-1 infection and the gastrointestinal tract reservoir.
P. D. Smith, G. Meng, J. F. Salazar-Gonzalez, and G. M. Shaw (2003)
J. Leukoc. Biol. 74, 642-649
   Abstract »    Full Text »    PDF »
Distinct Cycling CD4+- and CD8+-T-Cell Profiles during the Asymptomatic Phase of Simian Immunodeficiency Virus SIVmac251 Infection in Rhesus Macaques.
V. Monceaux, R. H. T. Fang, M. C. Cumont, B. Hurtrel, and J. Estaquier (2003)
J. Virol. 77, 10047-10059
   Abstract »    Full Text »    PDF »
Rapid and Irreversible CD4+ T-Cell Depletion Induced by the Highly Pathogenic Simian/Human Immunodeficiency Virus SHIVDH12R Is Systemic and Synchronous.
T. Igarashi, C. R. Brown, R. A. Byrum, Y. Nishimura, Y. Endo, R. J. Plishka, C. Buckler, A. Buckler-White, G. Miller, V. M. Hirsch, et al. (2002)
J. Virol. 76, 379-391
   Abstract »    Full Text »    PDF »
Unique Monoclonal Antibody Recognizing the Third Extracellular Loop of CXCR4 Induces Lymphocyte Agglutination and Enhances Human Immunodeficiency Virus Type 1-Mediated Syncytium Formation and Productive Infection.
R. Tanaka, A. Yoshida, T. Murakami, E. Baba, J. Lichtenfeld, T. Omori, T. Kimura, N. Tsurutani, N. Fujii, Z.-X. Wang, et al. (2001)
J. Virol. 75, 11534-11543
   Abstract »    Full Text »    PDF »
Human Immunodeficiency Virus 1 Envelope Glycoprotein Complex-Induced Apoptosis Involves Mammalian Target of Rapamycin/Fkbp12-Rapamycin-Associated Protein-Mediated P53 Phosphorylation.
M. Castedo, K. F. Ferri, J. Blanco, T. Roumier, N. Larochette, J. Barretina, A. Amendola, R. Nardacci, D. Metivier, J. A. Este, et al. (2001)
J. Exp. Med. 194, 1097-1110
   Abstract »    Full Text »    PDF »
Sodium Lauryl Sulfate Abrogates Human Immunodeficiency Virus Infectivity by Affecting Viral Attachment.
J. Bestman-Smith, J. Piret, A. Desormeaux, M. J. Tremblay, R. F. Omar, and M. G. Bergeron (2001)
Antimicrob. Agents Chemother. 45, 2229-2237
   Abstract »    Full Text »    PDF »
Partial Activation and Induction of Apoptosis in CD4+ and CD8+ T Lymphocytes by Conformationally Authentic Noninfectious Human Immunodeficiency Virus Type 1.
M. T. Esser, J. W. Bess Jr., K. Suryanarayana, E. Chertova, D. Marti, M. Carrington, L. O. Arthur, and J. D. Lifson (2001)
J. Virol. 75, 1152-1164
   Abstract »    Full Text »    PDF »
Resistance to Apoptosis in HIV-Infected CD4+ T Lymphocytes Is Mediated by Macrophages: Role for Nef and Immune Activation in Viral Persistence.
U. Mahlknecht, C. Deng, M. C. Lu, T. C. Greenough, J. L. Sullivan, W. A. O'Brien, and G. Herbein (2000)
J. Immunol. 165, 6437-6446
   Abstract »    Full Text »    PDF »
Apoptosis Control in Syncytia Induced by the HIV Type 1-Envelope Glycoprotein Complex: Role of Mitochondria and Caspases.
K. F. Ferri, E. Jacotot, J. Blanco, J. A. Este, N. Zamzami, S. A. Susin, Z. Xie, G. Brothers, J. C. Reed, J. M. Penninger, et al. (2000)
J. Exp. Med. 192, 1081-1092
   Abstract »    Full Text »    PDF »
Destruction of primary CD4+ T cells by cell-cell interaction in human immunodeficiency virus type 1 infection in vitro.
H. Stocker, C. Scheller, and C. Jassoy (2000)
J. Gen. Virol. 81, 1907-1911
   Abstract »    Full Text »
Tumor Necrosis Factor (TNF)-{alpha} and TNF Receptors in Viral Pathogenesis.
G. Herbein and W. A. O'brien (2000)
Experimental Biology and Medicine 223, 241-257
   Abstract »    Full Text »
The potential importance of HIV-induction of lymphocyte homing to lymph nodes.
J. J.-Y. Chen and M. W. Cloyd (1999)
Int. Immunol. 11, 1591-1594
   Abstract »    Full Text »    PDF »
Cyanovirin-N Binds to gp120 To Interfere with CD4-Dependent Human Immunodeficiency Virus Type 1 Virion Binding, Fusion, and Infectivity but Does Not Affect the CD4 Binding Site on gp120 or Soluble CD4-Induced Conformational Changes in gp120.
M. T. Esser, T. Mori, I. Mondor, Q. J. Sattentau, B. Dey, E. A. Berger, M. R. Boyd, and J. D. Lifson (1999)
J. Virol. 73, 4360-4371
   Abstract »    Full Text »    PDF »
Packaging Cell Lines for Simian Foamy Virus Type 1 Vectors.
M. Wu and A. Mergia (1999)
J. Virol. 73, 4498-4501
   Abstract »    Full Text »    PDF »
HIV-induced T-cell syncytia release a two component T-helper cell chemoattractant composed of Nef and Tat.
D. Shutt and D. Soll (1999)
J. Cell Sci. 112, 3931-3941
   Abstract »    PDF »
CD4+ T-Lymphocyte Depletion in Human Lymphoid Tissue Ex Vivo Is Not Induced by Noninfectious Human Immunodeficiency Virus Type 1 Virions.
A. W. Sylwester, J.-C. Grivel, W. Fitzgerald, J. L. Rossio, J. D. Lifson, and L. B. Margolis (1998)
J. Virol. 72, 9345-9347
   Abstract »    Full Text »    PDF »
Modulation of Human Immunodeficiency Virus Type 1-Induced Syncytium Formation by the Conformational State of LFA-1 Determined by a New Luciferase-Based Syncytium Quantitative Assay.
B. Barbeau, J.-F. Fortin, N. Genois, and M. J. Tremblay (1998)
J. Virol. 72, 7125-7136
   Abstract »    Full Text »    PDF »
Distinct Mechanisms Trigger Apoptosis in Human Immunodeficiency Virus Type 1-Infected and in Uninfected Bystander T Lymphocytes.
G. Herbein, C. Van Lint, J. L. Lovett, and E. Verdin (1998)
J. Virol. 72, 660-670
   Abstract »    Full Text »    PDF »
T cell syncytia induced by HIV release. T cell chemoattractants: demonstration with a newly developed single cell chemotaxis chamber.
D. Shutt, L. Jenkins, E. Carolan, J Stapleton, K. Daniels, R. Kennedy, and D. Soll (1998)
J. Cell Sci. 111, 99-109
   Abstract »    PDF »
The Immunopathogenesis of Human Immunodeficiency Virus Infection.
G. Pantaleo, C. Graziosi, and A. S. Fauci (1993)
N. Engl. J. Med. 328, 327-335
   Full Text »
HIV-induced syncytia of a T cell line form single giant pseudopods and are motile.
A Sylwester, D Wessels, S. Anderson, R. Warren, D. Shutt, R. Kennedy, and D. Soll (1993)
J. Cell Sci. 106, 941-953
   Abstract »    PDF »
Dendritic cells exposed to human immunodeficiency virus type-1 transmit a vigorous cytopathic infection to CD4+ T cells.
P. Cameron, P. Freudenthal, J. Barker, S Gezelter, K Inaba, and R. Steinman (1992)
Science 257, 383-387
   Abstract »    PDF »
Inhibition of entry of HIV-1 in neural cell lines by antibodies against galactosyl ceramide.
J. Harouse, S Bhat, S. Spitalnik, M Laughlin, K Stefano, D. Silberberg, and F Gonzalez-Scarano (1991)
Science 253, 320-323
   Abstract »    PDF »
Enhancement of HIV-1 cytocidal effects in CD4+ lymphocytes by the AIDS-associated mycoplasma.
S. Lo, S Tsai, Benish JR, J. Shih, D. Wear, and D. Wong (1991)
Science 251, 1074-1076
   Abstract »    PDF »
Biological Agents in Infectious Diseases.
S. G. Louie and G. S. Jaresko (1991)
Journal of Pharmacy Practice 4, 326-341
   Abstract »    PDF »
Activators of protein kinase C induce dissociation of CD4, but not CD8, from p56lck.
T. Hurley, K Luo, and B. Sefton (1989)
Science 245, 407-409
   Abstract »    PDF »
Unexpectedly high levels of HIV-1 RNA and protein synthesis in a cytocidal infection.
M Somasundaran and H. Robinson (1988)
Science 242, 1554-1557
   Abstract »    PDF »
Synthetic CD4 peptide derivatives that inhibit HIV infection and cytopathicity.
J. Lifson, K. Hwang, P. Nara, B Fraser, M Padgett, N. Dunlop, and L. Eiden (1988)
Science 241, 712-716
   Abstract »    PDF »
West African HIV-2-related human retrovirus with attenuated cytopathicity.
L. Kong, S. Lee, J. Kappes, J. Parkin, D Decker, J. Hoxie, B. Hahn, and G. Shaw (1988)
Science 240, 1525-1529
   Abstract »    PDF »
Dextran sulfate suppression of viruses in the HIV family: inhibition of virion binding to CD4+ cells.
H Mitsuya, D. Looney, S Kuno, R Ueno, F Wong-Staal, and S Broder (1988)
Science 240, 646-649
   Abstract »    PDF »
The human immunodeficiency virus: infectivity and mechanisms of pathogenesis.
A. Fauci (1988)
Science 239, 617-622
   Abstract »    PDF »
A quantitative bioassay for HIV-1 based on trans-activation.
B. Felber and G. Pavlakis (1988)
Science 239, 184-187
   Abstract »    PDF »
Blocking of HIV-1 infectivity by a soluble, secreted form of the CD4 antigen.
D. Smith, R. Byrn, S. Marsters, T Gregory, J. Groopman, and D. Capon (1987)
Science 238, 1704-1707
   Abstract »    PDF »
HTLV-III/LAV-neutralizing antibodies to an E. coli-produced fragment of the virus envelope.
S. Putney, T. Matthews, W. Robey, D. Lynn, M Robert-Guroff, W. Mueller, A. Langlois, J Ghrayeb, S. Petteway Jr, K. Weinhold, et al. (1986)
Science 234, 1392-1395
   Abstract »    PDF »
Alterations in T4 (CD4) protein and mRNA synthesis in cells infected with HIV.
J. Hoxie, J. Alpers, J. Rackowski, K Huebner, B. Haggarty, A. Cedarbaum, and J. Reed (1986)
Science 234, 1123-1127
   Abstract »    PDF »
HIV Nef-mediated Cellular Phenotypes Are Differentially Expressed as a Function of Intracellular NEF Concentrations.
X. Liu, J. A. Schrager, G. D. Lange, and J. W. Marsh (2001)
J. Biol. Chem. 276, 32763-32770
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)