Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 9 May 1986:
Vol. 232. no. 4751, pp. 768 - 772
DOI: 10.1126/science.3008339
Prev | Table of Contents | Next

Articles

Science, Vol 232, Issue 4751, 768-772
Copyright © 1986 by American Association for the Advancement of Science

articles

Identification and characterization of the protein encoded by the human N-myc oncogene

DJ Slamon, TC Boone, RC Seeger, DE Keith, V Chazin, HC Lee, and LM Souza

The human N-myc gene is related to the c-myc proto-oncogene, and has been shown to have transforming potential in vitro. Many studies have reported amplification of N-myc in human neuroblastoma and retinoblastoma cell lines. In primary tumors, amplification of the gene was found to correlate directly with behavior of the tumor. Specific restriction fragments of a partial complementary DNA clone of N-myc from LA-N-5 human neuroblastoma cells were placed into a bacterial expression vector for the purpose of producing antigens representative of the N-myc protein. Rabbits immunized with these antigens produced antisera that recognized a protein of 62-64 kilodaltons in neuroblastoma cells. By several criteria, this protein appears to be part of the same proto-oncogene family as the c-myc protein. Moreover, the antisera to fragments of this protein were capable of histochemically identifying malignant cells in clinical specimens.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Natural Killer T Cells Infiltrate Neuroblastomas Expressing the Chemokine CCL2.
L. S. Metelitsa, H.-W. Wu, H. Wang, Y. Yang, Z. Warsi, S. Asgharzadeh, S. Groshen, S. B. Wilson, and R. C. Seeger (2004)
J. Exp. Med. 199, 1213-1221
   Abstract »    Full Text »    PDF »
The Expression of the Developmentally Regulated Proto-oncogene Pax-3 Is Modulated by N-Myc.
R. G. Harris, E. White, E. S. Phillips, and K. A. Lillycrop (2002)
J. Biol. Chem. 277, 34815-34825
   Abstract »    Full Text »    PDF »
Inhibition of N-myc Expression and Induction of Apoptosis by Iron Chelation in Human Neuroblastoma Cells.
L. Fan, J. Iyer, S. Zhu, K. K. Frick, R. K. Wada, A. E. Eskenazi, P. E. Berg, N. Ikegaki, R. H. Kennett, and C. N. Frantz (2001)
Cancer Res. 61, 1073-1079
   Abstract »    Full Text »
MYCN Expression in Neuroblastoma: A Mixed Message?.
K. K. Matthay (2000)
J. Clin. Oncol. 18, 3591-3594
   Full Text »    PDF »
Molecular Biology of Neuroblastoma.
J. M. Maris and K. K. Matthay (1999)
J. Clin. Oncol. 17, 2264
   Abstract »    Full Text »    PDF »
A Novel Intron Element Operates Posttranscriptionally To Regulate Human N-myc Expression.
L. E. Sivak, G. Pont-Kingdon, K. Le, G. Mayr, K.-F. Tai, B. T. Stevens, and W. L. Carroll (1999)
Mol. Cell. Biol. 19, 155-163
   Abstract »    Full Text »    PDF »
Defects of embryonic organogenesis resulting from targeted disruption of the N-myc gene in the mouse.
S Sawai, A Shimono, Y Wakamatsu, C Palmes, K Hanaoka, and H Kondoh (1993)
Development 117, 1445-1455
   Abstract »    PDF »
Loss of N-myc function results in embryonic lethality and failure of the epithelial component of the embryo to develop..
B R Stanton, A S Perkins, L Tessarollo, D A Sassoon, and L F Parada (1992)
Genes & Dev. 6, 2235-2247
   Abstract »    PDF »
Contrasting patterns of c-myc and N-myc expression in proliferating, quiescent, and differentiating cells of the embryonic chicken lens.
L. Harris, J. Talian, and P. Zelenka (1992)
Development 115, 813-820
   Abstract »    PDF »
The Role of myc Oncogenes in Cell Growth and Differentiation.
T.D. Littlewood and G.I. Evan (1990)
Advances in Dental Research 4, 69-79
   Abstract »    PDF »
Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer.
D. Slamon, W Godolphin, L. Jones, J. Holt, S. Wong, D. Keith, W. Levin, S. Stuart, J Udove, A Ullrich, et al. (1989)
Science 244, 707-712
   Abstract »    PDF »
The Stases in Scientific and Literary Argument.
J. FAHNESTOCK and M. SECOR (1988)
Written Communication 5, 427-443
   Abstract »
The human myc gene family: structure and activity of L-myc and an L-myc pseudogene..
R A DePinho, K S Hatton, A Tesfaye, G D Yancopoulos, and F W Alt (1987)
Genes & Dev. 1, 1311-1326
   Abstract »    PDF »
The Human myc Gene Family.
F.W. Alt, R. DePinho, K. Zimmerman, E. Legouy, K. Hatton, P. Ferrier, A. Tesfaye, G. Yancopoulos, and P. Nisen (1986)
Cold Spring Harb Symp Quant Biol 51, 931-941
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)