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Science 25 April 1986:
Vol. 232. no. 4749, pp. 525 - 528
DOI: 10.1126/science.3961495
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Articles

Science, Vol 232, Issue 4749, 525-528
Copyright © 1986 by American Association for the Advancement of Science

articles

A fluorescence photobleaching assay of gap junction-mediated communication between human cells

MH Wade, JE Trosko, and M Schindler

Gap junction-mediated communication between contiguous cells has been implicated in the regulation of cell proliferation and differentiation. This report describes a new technique to measure cell-cell communication, gap fluorescence redistribution after photobleaching, which is based on the diffusion-dependent return of 6-carboxyfluorescein-mediated fluorescence in a photobleached cell that is in contact with other fluorescently labeled cells. Fluorescence recovery rates are interpreted as dye transport across gap junctions. Results of experiments on normal human fibroblasts and human teratocarcinoma cells show that this technique can measure rapid dye transfer and detect inhibition of communication (between teratocarcinoma cells) by the tumor promoters 12-O-tetradecanoyl-phorbol-13-acetate and the pesticide dieldrin.


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