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Science 25 April 1986:
Vol. 232. no. 4749, pp. 518 - 521
DOI: 10.1126/science.3008331
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Articles

Science, Vol 232, Issue 4749, 518-521
Copyright © 1986 by American Association for the Advancement of Science

articles

Gene transfer and molecular cloning of the human NGF receptor

MV Chao, MA Bothwell, AH Ross, H Koprowski, AA Lanahan, CR Buck, and A Sehgal

Nerve growth factor (NGF) and its receptor are important in the development of cells derived from the neural crest. Mouse L cell transformants have been generated that stably express the human NGF receptor gene transfer with total human DNA. Affinity cross-linking, metabolic labeling and immunoprecipitation, and equilibrium binding with 125I-labeled NGF revealed that this NGF receptor had the same size and binding characteristics as the receptor from human melanoma cells and rat PC12 cells. The sequences encoding the NGF receptor were molecularly cloned using the human Alu repetitive sequence as a probe. A cosmid clone that contained the human NGF receptor gene allowed efficient transfection and expression of the receptor.


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