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Science 25 April 1986:
Vol. 232. no. 4749, pp. 503 - 506
DOI: 10.1126/science.2421410
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Articles

Science, Vol 232, Issue 4749, 503-506
Copyright © 1986 by American Association for the Advancement of Science

articles

Restoration by calmodulin of a Ca2+-dependent K+ current missing in a mutant of Paramecium

RD Hinrichsen, A Burgess-Cassler, BC Soltvedt, T Hennessey, and C Kung

A combination of genetics, biochemistry, and biophysics was used to show that calmodulin is involved in the regulation of an ion channel. Calmodulin restored the Ca2+-dependent K+ current in pantophobiac, a mutant in Paramecium that lacks this current. The restoration of the current occurred within 2 hours after the injection of 1 picogram of wild-type calmodulin into the mutant. The current remained for approximately 30 hours before the mutant phenotype returned. The injection of calmodulin isolated from pantophobiac had no effect. These results imply that calmodulin is required for the function or regulation of the Ca2+-dependent K+ current in Paramecium.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Calmodulin Regulates Synaptic Plasticity in the Anterior Cingulate Cortex and Behavioral Responses: A Microelectroporation Study in Adult Rodents.
F. Wei, X.-M. Xia, J. Tang, H. Ao, S. Ko, J. Liauw, C.-S. Qiu, and M. Zhuo (2003)
J. Neurosci. 23, 8402-8409
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K+-Channel Transgenes Reduce K+ Currents in Paramecium, Probably by a Post-translational Mechanism.
K.-Y. Ling, W. J. Haynes, L. Oesterle, C. Kung, R. R. Preston, and Y. Saimi (2001)
Genetics 159, 987-995
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Regulation of Ca2+-activated K+ channels by multifunctional Ca2+/calmodulin-dependent protein kinase.
S. C. Sansom, R. Ma, P. K. Carmines, and D. A. Hall (2000)
Am J Physiol Renal Physiol 279, F283-F288
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Calmodulin activation of calcium-dependent sodium channels in excised membrane patches of Paramecium.
Y Saimi and K. Ling (1990)
Science 249, 1441-1444
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Ion Channels in Paramecium, Yeast, and Escherichia coli.
Y. Saimi, B. Martinac, M.C. Gustin, M.R. Culbertson, J. Adler, and C. Kung (1988)
Cold Spring Harb Symp Quant Biol 53, 667-673
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