Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 4 April 1986:
Vol. 232. no. 4746, pp. 97 - 100
DOI: 10.1126/science.3006254
Prev | Table of Contents | Next

Articles

Science, Vol 232, Issue 4746, 97-100
Copyright © 1986 by American Association for the Advancement of Science

articles

Receptor-coupled activation of phosphoinositide-specific phospholipase C by an N protein

CD Smith, CC Cox, and R Snyderman

Cleavage of phosphatidylinositol 4,5-bisphosphate by phospholipase C results in the production of two important second messengers: inositol-1,4,5-trisphosphate and 1,2-diacylglycerol. Although several receptors promote this cleavage, the molecular details of phospholipase C activation have remained unresolved. In this study, occupancy of a Ca2+-mobilizing receptor, the oligopeptide chemoattractant receptor on human polymorphonuclear leukocyte plasma membranes, was found to lead to the activation of a guanine nucleotide regulatory (N) protein by guanosine 5'-triphosphate. The activated N protein then stimulated a polyphosphoinositide-specific phospholipase C by reducing the Ca2+ requirement for expression of this activity from superphysiological to normal intracellular concentrations. Therefore, the N protein-mediated activation of phospholipase C may be a key step in the pathway of cellular activation by chemoattractants and certain other hormones.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Depletion of {beta}-Arrestin-2 Promotes Tumor Growth and Angiogenesis in a Murine Model of Lung Cancer.
S. K. Raghuwanshi, M. W. Nasser, X. Chen, R. M. Strieter, and R. M. Richardson (2008)
J. Immunol. 180, 5699-5706
   Abstract »    Full Text »    PDF »
Altered CXCR2 Signaling in {beta}-Arrestin-2-Deficient Mouse Models.
Y. Su, S. K. Raghuwanshi, Y. Yu, L. B. Nanney, R. M. Richardson, and A. Richmond (2005)
J. Immunol. 175, 5396-5402
   Abstract »    Full Text »    PDF »
Urokinase-deficient and urokinase receptor-deficient mice have impaired neutrophil antimicrobial activation in vitro.
M. R. Gyetko, D. Aizenberg, and L. Mayo-Bond (2004)
J. Leukoc. Biol. 76, 648-656
   Abstract »    Full Text »    PDF »
The Pleckstrin Homology Domain of Phospholipase C-{beta}2 as an Effector Site for Rac.
J. T. Snyder, A. U. Singer, M. R. Wing, T. K. Harden, and J. Sondek (2003)
J. Biol. Chem. 278, 21099-21104
   Abstract »    Full Text »    PDF »
Chemoattractant Receptor Cross-desensitization.
H. Ali, R. M. Richardson, B. Haribabu, and R. Snyderman (1999)
J. Biol. Chem. 274, 6027-6030
   Full Text »    PDF »
Phospholipase C beta  and Membrane Action of Calcitriol and Estradiol.
V. Le Mellay, B. Grosse, and M. Lieberherr (1997)
J. Biol. Chem. 272, 11902-11907
   Abstract »    Full Text »    PDF »
Signal Transduction by the Formyl Peptide Receptor.
T. T. Amatruda III, S. Dragas-Graonic, R. Holmes, and H. D. Perez (1995)
J. Biol. Chem. 270, 28010-28013
   Abstract »    Full Text »    PDF »
A 32-kD GTP-binding protein associated with the CD4-p56lck and CD8-p56lck T cell receptor complexes.
J. Telfer and C. Rudd (1991)
Science 254, 439-441
   Abstract »    PDF »
Studies of inositol phospholipid-specific phospholipase C.
S. Rhee, P. Suh, S. Ryu, and S. Lee (1989)
Science 244, 546-550
   Abstract »    PDF »
Primary Structure of PLC-154.
M. Katan, R.W. Kriz, N. Totty, R. Philp, E. Meldrum, R.A. Aldape, J. Knopf, and R.J. Parker (1988)
Cold Spring Harb Symp Quant Biol 53, 921-926
   Abstract »    PDF »
GTP-binding Proteins in the Control of Exocytosis.
B.D. Gomperts and P.E.R. Tatham (1988)
Cold Spring Harb Symp Quant Biol 53, 983-992
   Abstract »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)