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Science 28 March 1986:
Vol. 231. no. 4745, pp. 1577 - 1580
DOI: 10.1126/science.231.4745.1577
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Articles

Autolytic Processing of Dimeric Plant Virus Satellite RNA

GERRY A. PRODY 1, JOHN T. BAKOS 2, JAMAL M. BUZAYAN 2, IRVING R. SCHNEIDER 2, and GEORGE BRUENING 2

1 Department of Chemistry, Western Washington University, Bellingham, WA 98225.
2 Department of Plant Pathology, College of Agricultural and Environmental Sciences, University of California, Davis, CA 95616.

Associated with some plant viruses are small satellite RNA's that depend on the plant virus to provide protective coat protein and presumably at least some of the proteins necessary for satellite RNA replication. Multimeric forms of the satellite RNA of tobacco ringspot virus are probable in vivo precursors of the monomeric satellite RNA. Evidence is presented for the in vitro autolytic processing of dimeric and trimeric forms of this satellite RNA. The reaction generates biologically active monomeric satellite RNA, apparently is reversible to form dimeric RNA from monomeric RNA, and does not require an enzyme for its catalysis.

Submitted on May 30, 1985
Accepted on December 16, 1985


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