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Science 21 March 1986:
Vol. 231. no. 4744, pp. 1401 - 1406
DOI: 10.1126/science.3952490
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Articles

Science, Vol 231, Issue 4744, 1401-1406
Copyright © 1986 by American Association for the Advancement of Science

articles

Structure of tobacco mosaic virus at 3.6 A resolution: implications for assembly

K Namba and G Stubbs

X-ray fiber diffraction analysis of tobacco mosaic virus (TMV) has led to the building of a molecular model of the intact virus, based on a map at 3.6 A resolution derived from five separated Bessel orders. This has been made possible by advances in the solution of the fiber diffraction phase problem. It is now possible to understand much of the chemical basis of TMV assembly, particularly in terms of intersubunit electrostatic interactions and RNA binding. Consideration of the molecular structure in conjunction with physical chemical studies by several groups of investigators suggests that the nucleating aggregate for initiation of TMV assembly is a short (about two turns) helix of protein subunits, probably inhibited from further polymerization in the absence of RNA by the disordering of peptide loop near the inner surface of the virus.


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