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Science 7 March 1986: Vol. 231. no. 4742, pp. 1150 - 1154 DOI: 10.1126/science.3753802
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Articles
Science, Vol 231, Issue 4742, 1150-1154
Copyright © 1986 by American Association for the Advancement of Science
Sequence and expression of human estrogen receptor complementary DNA
GL Greene,
P Gilna,
M Waterfield,
A Baker,
Y Hort,
and
J Shine
The mechanism by which the estrogen receptor and other steroid hormone receptors regulate gene expression in eukaryotic cells is not well understood. In this study, a complementary DNA clone containing the entire translated portion of the messenger RNA for the estrogen receptor from MCF-7 human breast cancer cells was sequenced and then expressed in Chinese hamster ovary (CHO-K1) cells to give a functional protein. An open reading frame of 1785 nucleotides in the complementary DNA corresponded to a polypeptide of 595 amino acids and a molecular weight of 66,200, which is in good agreement with published molecular weight values of 65,000 to 70,000 for the estrogen receptor. Homogenates of transformed Chinese hamster ovary cells containing a protein that bound [3H]estradiol and sedimented as a 4S complex in salt-containing sucrose gradients and as an 8 to 9S complex in the absence of salt. Interaction of this receptor-[3H]estradiol complex with a monoclonal antibody that is specific for primate ER confirms the identity of the expressed complementary DNA as human estrogen receptor. Amino acid sequence comparisons revealed significant regional homology among the human estrogen receptor, the human glucocorticoid receptor, and the putative v-erbA oncogene product. This suggests that steroid receptor genes and the avian erythroblastosis viral oncogene are derived from a common primordial gene. The homologous region, which is rich in cysteine, lysine, and arginine, may represent the DNA-binding domain of these proteins.
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- Estrogen Receptor Isoform Gene Expression in Ovarian Stromal and Epithelial Tumors.
- S. Chu, P. Mamers, H. G. Burger, and P. J. Fuller (2000)
J. Clin. Endocrinol. Metab.
85, 1200-1205
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- Differences in Estrogen Receptor {{alpha}} Variant Messenger RNAs between Normal Human Breast Tissue and Primary Breast Carcinomas.
- M. A. J. van Dijk, A. A. M. Hart, and L. J. v.'t Veer (2000)
Cancer Res.
60, 530-533
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- Mouse Oviduct-Specific Glycoprotein Gene: Genomic Organization and Structure of the 5'-Flanking Regulatory Region.
- K. Takahashi, Y. Sendai, Y. Matsuda, H. Hoshi, M. Hiroi, and Y. Araki (2000)
Biol Reprod
62, 217-226
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- Expression Profiling of the Maize Flavonoid Pathway Genes Controlled by Estradiol-Inducible Transcription Factors CRC and P.
- W. Bruce, O. Folkerts, C. Garnaat, O. Crasta, B. Roth, and B. Bowen (2000)
PLANT CELL
12, 65-80
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- Estrogen and Progesterone Receptors: An Overview From the Year 2000.
- C. M. Peterson (2000)
Reproductive Sciences
7, S3-S7
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- Selective Estrogen Receptor Modulators (SERMs): A First Step in the Development of Perfect Hormone Replacement Therapy Regimen.
- D. P. McDonnell (2000)
Reproductive Sciences
7, S10-S15
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- Estrogen: Consequences and Implications of Human Mutations in Synthesis and Action.
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J. Clin. Endocrinol. Metab.
84, 4677-4694
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- A Fusion Protein of the Estrogen Receptor (ER) and Nuclear Receptor Corepressor (NCoR) Strongly Inhibits Estrogen-Dependent Responses in Breast Cancer Cells.
- P.-Y. Chien, M. Ito, Y. Park, T. Tagami, B. D. Gehm, and J. L. Jameson (1999)
Mol. Endocrinol.
13, 2122-2136
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- Estradiol Inhibits Leukocyte Adhesion and Transendothelial Migration in Rabbits In Vivo : Possible Mechanisms for Gender Differences in Atherosclerosis.
- L. Nathan, S. Pervin, R. Singh, M. Rosenfeld, and G. Chaudhuri (1999)
Circ. Res.
85, 377-385
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- Estrogen receptor-{alpha} gene transfer into bovine aortic endothelial cells induces eNOS gene expression and inhibits cell migration.
- E. Tan, M. V Gurjar, R. V Sharma, and R. C Bhalla (1999)
Cardiovasc Res
43, 788-797
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- Molecular Control of Immune/Inflammatory Responses: Interactions Between Nuclear Factor-{kappa}B and Steroid Receptor-Signaling Pathways.
- L. I. McKay and J. A. Cidlowski (1999)
Endocr. Rev.
20, 435-459
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- Tamoxifen, Raloxifene, and the Prevention of Breast Cancer.
- V. C. Jordan and M. Morrow (1999)
Endocr. Rev.
20, 253-278
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- Oestrogen receptor {alpha} and ฿ mRNA expression in human endometrium throughout the menstrual cycle.
- S. Matsuzaki, T. Fukaya, T. Suzuki, T. Murakami, H. Sasano, and A. Yajima (1999)
Mol. Hum. Reprod.
5, 559-564
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- Redox-dependent Regulation of Nuclear Import of the Glucocorticoid Receptor.
- K. Okamoto, H. Tanaka, H. Ogawa, Y. Makino, H. Eguchi, S.-i. Hayashi, N. Yoshikawa, L. Poellinger, K. Umesono, and I. Makino (1999)
J. Biol. Chem.
274, 10363-10371
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- HMG-1 Stimulates Estrogen Response Element Binding by Estrogen Receptor from Stably Transfected HeLa Cells.
- C. C. Zhang, S. Krieg, and D. J. Shapiro (1999)
Mol. Endocrinol.
13, 632-643
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- Coexpression of Alternatively Spliced Estrogen and Progesterone Receptor Transcripts in Human Breast Cancer.
- R. L. Balleine, S. M. N. Hunt, and C. L. Clarke (1999)
J. Clin. Endocrinol. Metab.
84, 1370-1377
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- Estrogen receptor (ER) modulators each induce distinct conformational changes in ER alpha and ER beta.
- L. A. Paige, D. J. Christensen, H. Gron, J. D. Norris, E. B. Gottlin, K. M. Padilla, C.-y. Chang, L. M. Ballas, P. T. Hamilton, D. P. McDonnell, et al. (1999)
PNAS
96, 3999-4004
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- Dominant Activity of Activation Function 1 (AF-1) and Differential Stoichiometric Requirements for AF-1 and -2 in the Estrogen Receptor alpha -beta Heterodimeric Complex.
- G. B. Tremblay, A. Tremblay, F. Labrie, and V. Giguere (1999)
Mol. Cell. Biol.
19, 1919-1927
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- Coexpression of Estrogen Receptor {{alpha}} and {beta}: Poor Prognostic Factors in Human Breast Cancer?.
- V. Speirs, A. T. Parkes, M. J. Kerin, D. S. Walton, P. J. Carleton, J. N. Fox, and S. L. Atkin (1999)
Cancer Res.
59, 525-528
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- AF-2-Dependent Potentiation of CCAAT Enhancer Binding Protein {beta}-Mediated Transcriptional Activation by Glucocorticoid Receptor.
- M. Boruk, J. G. A. Savory, and R. J. G. Haché (1998)
Mol. Endocrinol.
12, 1749-1763
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- Transcription Activation by the Human Estrogen Receptor Subtype {beta} (ER{beta}) Studied with ER{beta} and ER{alpha} Receptor Chimeras.
- E. M. McInerney, K. E. Weis, J. Sun, S. Mosselman, and B. S. Katzenellenbogen (1998)
Endocrinology
139, 4513-4522
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