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Science 7 March 1986:
Vol. 231. no. 4742, pp. 1150 - 1154
DOI: 10.1126/science.3753802

Articles

Science, Vol 231, Issue 4742, 1150-1154
Copyright © 1986 by American Association for the Advancement of Science


articles

Sequence and expression of human estrogen receptor complementary DNA

GL Greene, P Gilna, M Waterfield, A Baker, Y Hort, and J Shine

The mechanism by which the estrogen receptor and other steroid hormone receptors regulate gene expression in eukaryotic cells is not well understood. In this study, a complementary DNA clone containing the entire translated portion of the messenger RNA for the estrogen receptor from MCF-7 human breast cancer cells was sequenced and then expressed in Chinese hamster ovary (CHO-K1) cells to give a functional protein. An open reading frame of 1785 nucleotides in the complementary DNA corresponded to a polypeptide of 595 amino acids and a molecular weight of 66,200, which is in good agreement with published molecular weight values of 65,000 to 70,000 for the estrogen receptor. Homogenates of transformed Chinese hamster ovary cells containing a protein that bound [3H]estradiol and sedimented as a 4S complex in salt-containing sucrose gradients and as an 8 to 9S complex in the absence of salt. Interaction of this receptor-[3H]estradiol complex with a monoclonal antibody that is specific for primate ER confirms the identity of the expressed complementary DNA as human estrogen receptor. Amino acid sequence comparisons revealed significant regional homology among the human estrogen receptor, the human glucocorticoid receptor, and the putative v-erbA oncogene product. This suggests that steroid receptor genes and the avian erythroblastosis viral oncogene are derived from a common primordial gene. The homologous region, which is rich in cysteine, lysine, and arginine, may represent the DNA-binding domain of these proteins.


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G. I. Gorodeski (2001)
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Functional Differences between the Amino-Terminal Domains of Estrogen Receptors alpha and beta.
F. Delaunay, K. Pettersson, M. Tujague, and J.-A. Gustafsson (2000)
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Involvement of estrogen receptors alpha and beta in the regulation of cervical permeability.
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J. Clin. Endocrinol. Metab. 84, 4677-4694
   Abstract »    Full Text »
A Fusion Protein of the Estrogen Receptor (ER) and Nuclear Receptor Corepressor (NCoR) Strongly Inhibits Estrogen-Dependent Responses in Breast Cancer Cells.
P.-Y. Chien, M. Ito, Y. Park, T. Tagami, B. D. Gehm, and J. L. Jameson (1999)
Mol. Endocrinol. 13, 2122-2136
   Abstract »    Full Text »
Estradiol Inhibits Leukocyte Adhesion and Transendothelial Migration in Rabbits In Vivo : Possible Mechanisms for Gender Differences in Atherosclerosis.
L. Nathan, S. Pervin, R. Singh, M. Rosenfeld, and G. Chaudhuri (1999)
Circ. Res. 85, 377-385
   Abstract »    Full Text »    PDF »
Estrogen receptor-{alpha} gene transfer into bovine aortic endothelial cells induces eNOS gene expression and inhibits cell migration.
E. Tan, M. V Gurjar, R. V Sharma, and R. C Bhalla (1999)
Cardiovasc Res 43, 788-797
   Abstract »    Full Text »    PDF »
Molecular Control of Immune/Inflammatory Responses: Interactions Between Nuclear Factor-{kappa}B and Steroid Receptor-Signaling Pathways.
L. I. McKay and J. A. Cidlowski (1999)
Endocr. Rev. 20, 435-459
   Abstract »    Full Text »    PDF »
Tamoxifen, Raloxifene, and the Prevention of Breast Cancer.
V. C. Jordan and M. Morrow (1999)
Endocr. Rev. 20, 253-278
   Abstract »    Full Text »
Oestrogen receptor {alpha} and ฿ mRNA expression in human endometrium throughout the menstrual cycle.
S. Matsuzaki, T. Fukaya, T. Suzuki, T. Murakami, H. Sasano, and A. Yajima (1999)
Mol. Hum. Reprod. 5, 559-564
   Abstract »    Full Text »    PDF »
Redox-dependent Regulation of Nuclear Import of the Glucocorticoid Receptor.
K. Okamoto, H. Tanaka, H. Ogawa, Y. Makino, H. Eguchi, S.-i. Hayashi, N. Yoshikawa, L. Poellinger, K. Umesono, and I. Makino (1999)
J. Biol. Chem. 274, 10363-10371
   Abstract »    Full Text »    PDF »
HMG-1 Stimulates Estrogen Response Element Binding by Estrogen Receptor from Stably Transfected HeLa Cells.
C. C. Zhang, S. Krieg, and D. J. Shapiro (1999)
Mol. Endocrinol. 13, 632-643
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Coexpression of Alternatively Spliced Estrogen and Progesterone Receptor Transcripts in Human Breast Cancer.
R. L. Balleine, S. M. N. Hunt, and C. L. Clarke (1999)
J. Clin. Endocrinol. Metab. 84, 1370-1377
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Estrogen receptor (ER) modulators each induce distinct conformational changes in ER alpha  and ER beta.
L. A. Paige, D. J. Christensen, H. Gron, J. D. Norris, E. B. Gottlin, K. M. Padilla, C.-y. Chang, L. M. Ballas, P. T. Hamilton, D. P. McDonnell, et al. (1999)
PNAS 96, 3999-4004
   Abstract »    Full Text »    PDF »
Dominant Activity of Activation Function 1 (AF-1) and Differential Stoichiometric Requirements for AF-1 and -2 in the Estrogen Receptor alpha -beta Heterodimeric Complex.
G. B. Tremblay, A. Tremblay, F. Labrie, and V. Giguere (1999)
Mol. Cell. Biol. 19, 1919-1927
   Abstract »    Full Text »    PDF »
Coexpression of Estrogen Receptor {{alpha}} and {beta}: Poor Prognostic Factors in Human Breast Cancer?.
V. Speirs, A. T. Parkes, M. J. Kerin, D. S. Walton, P. J. Carleton, J. N. Fox, and S. L. Atkin (1999)
Cancer Res. 59, 525-528
   Abstract »    Full Text »    PDF »
AF-2-Dependent Potentiation of CCAAT Enhancer Binding Protein {beta}-Mediated Transcriptional Activation by Glucocorticoid Receptor.
M. Boruk, J. G. A. Savory, and R. J. G. Haché (1998)
Mol. Endocrinol. 12, 1749-1763
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Transcription Activation by the Human Estrogen Receptor Subtype {beta} (ER{beta}) Studied with ER{beta} and ER{alpha} Receptor Chimeras.
E. M. McInerney, K. E. Weis, J. Sun, S. Mosselman, and B. S. Katzenellenbogen (1998)
Endocrinology 139, 4513-4522
   Abstract »    Full Text »    PDF »



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