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Science 7 March 1986:
Vol. 231. no. 4742, pp. 1141 - 1145
DOI: 10.1126/science.3003919
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Articles

Science, Vol 231, Issue 4742, 1141-1145
Copyright © 1986 by American Association for the Advancement of Science

articles

Endonucleolytic activity that cleaves immunoglobulin recombination sequences

TJ Hope, RJ Aguilera, ME Minie, and H Sakano

An endonucleolytic activity has been identified in nuclear extracts of chick embryo bursa and mouse fetal liver cells. The activity introduces a double-strand cut in the vicinity of the recombination site of immunoglobulin joining gene segments. The cleavage occurs at the dinucleotide pair TG-AC. This activity is a good candidate for the putative endonuclease involved in recombination of the immunoglobulin variable, diversity, and joining regions. It is distinct from the endonuclease activities previously reported by others.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Biased VH Gene Usage in Early Lineage Human B Cells: Evidence for Preferential Ig Gene Rearrangement in the Absence of Selection.
S. P. Rao, J. M. Riggs, D. F. Friedman, M. S. Scully, T. W. LeBien, and L. E. Silberstein (1999)
J. Immunol. 163, 2732-2740
   Abstract »    Full Text »    PDF »
Two pairs of recombination signals are sufficient to cause immunoglobulin V-(D)-J joining.
S Akira, K Okazaki, and H Sakano (1987)
Science 238, 1134-1138
   Abstract »    PDF »
Early restriction of the human antibody repertoire.
H. Schroeder Jr, J. Hillson, and R. Perlmutter (1987)
Science 238, 791-793
   Abstract »    PDF »
Programmed gene rearrangements altering gene expression.
P Borst and D. Greaves (1987)
Science 235, 658-667
   Abstract »    PDF »


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