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Science 14 February 1986:
Vol. 231. no. 4739, pp. 737 - 740
DOI: 10.1126/science.3003910
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Articles

Science, Vol 231, Issue 4739, 737-740
Copyright © 1986 by American Association for the Advancement of Science

articles

High titers of autoantibodies to topoisomerase I (Scl-70) in sera from scleroderma patients

JH Shero, B Bordwell, NF Rothfield, and WC Earnshaw

Patients with rheumatic diseases often have circulating autoantibodies to nuclear components. The clinical significance of the antibodies is controversial, although in some cases they are valuable in the diagnosis of the disease. This report presents results of a study of Scl-70, an autoantigen recognized by sera of many patients with the most severe form of progressive systemic sclerosis. It was possible to show, by three independent criteria, that Scl-70 is the abundant nuclear enzyme DNA topoisomerase I. Therefore, antibody probes of high titer and high affinity are now available for the study of this important nuclear enzyme.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Mitotic Phosphorylation Stimulates DNA Relaxation Activity of Human Topoisomerase I.
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Molecular Recognition Patterns of Serum Anti-DNA Topoisomerase I Antibody in Systemic Sclerosis.
P. Q. Hu, N. Fertig, T. A. Medsger Jr, and T. M. Wright (2004)
J. Immunol. 173, 2834-2841
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ALTERED AUTOANTIGEN STRUCTURE IN SJoGREN'S SYNDROME: IMPLICATIONS FOR THE PATHOGENESIS OF AUTOIMMUNE TISSUE DAMAGE.
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Critical Reviews in Oral Biology & Medicine 15, 156-164
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Hydrogen Peroxide Induces Topoisomerase I-mediated DNA Damage and Cell Death.
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Identification of the Autoantigen HB as the Barrier-to-Autointegration Factor.
I. Forne, M. Carrascal, L. Martinez-Lostao, J. Abian, J. L. Rodriguez-Sanchez, and C. Juarez (2003)
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Hoechst 33342-Induced Apoptosis is Associated with Decreased Immunoreactive Topoisomerase I and Topoisomerase I-DNA Complex Formation.
X. Zhang and F. Kiechle (2001)
Ann. Clin. Lab. Sci. 31, 187-198
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Sensitivity and Specificity of Immunological Methods for the Detection of Anti-Topoisomerase I (Scl70) Autoantibodies: Results of a Multicenter Study.
N. Bizzaro, E. Tonutti, D. Villalta, D. Bassetti, R. Tozzoli, F. Manoni, S. Pirrone, A. Piazza, P. Rizzotti, M. Pradella, et al. (2000)
Clin. Chem. 46, 1681-1685
   Abstract »    Full Text »    PDF »
Analysis of Soluble and Cell Surface Factors Regulating Anti-DNA Topoisomerase I Autoantibody Production Demonstrates Synergy Between Th1 and Th2 Autoreactive T Cells.
M. Kuwana, T. A. Medsger Jr., and T. M. Wright (2000)
J. Immunol. 164, 6138-6146
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In vivo cell penetration and intracellular transport of anti-Sm and anti-La autoantibodies.
S. X. Deng, E. Hanson, and I. Sanz (2000)
Int. Immunol. 12, 415-423
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Is Anti-Topoisomerase I a Serum Marker of Pulmonary Involvement in Systemic Sclerosis?.
E. Diot, B. Giraudeau, P. Diot, D. Degenne, L. Ritz, J.-L. Guilmot, and E. Lemarie (1999)
Chest 116, 715-720
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Activity and Pharmacodynamics of 21-Day Topotecan Infusion in Patients With Ovarian Cancer Previously Treated With Platinum-Based Chemotherapy.
H. Hochster, S. Wadler, C. Runowicz, L. Liebes, H. Cohen, R. Wallach, J. Sorich, B. Taubes, and J. Speyer (1999)
J. Clin. Oncol. 17, 2553
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Cytogenetic study and FISH analysis in lymphocytes of systemic lupus erythematosus (SLE) and systemic sclerosis (SS) patients.
L. Migliore, C. Bevilacqua, and R. Scarpato (1999)
Mutagenesis 14, 227-231
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Caspase-mediated Cleavage of DNA Topoisomerase I at Unconventional Sites during Apoptosis.
K. Samejima, P. A. Svingen, G. S. Basi, T. Kottke, P. W. Mesner Jr., L. Stewart, F. Durrieu, G. G. Poirier, E. S. Alnemri, J. J. Champoux, et al. (1999)
J. Biol. Chem. 274, 4335-4340
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Ubiquitin-dependent Destruction of Topoisomerase I Is Stimulated by the Antitumor Drug Camptothecin.
S. D. Desai, L. F. Liu, D. Vazquez-Abad, and P. D'Arpa (1997)
J. Biol. Chem. 272, 24159-24164
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Scleroderma Autoantigens Are Uniquely Fragmented by Metal-catalyzed Oxidation Reactions: Implications for Pathogenesis.
L. Casciola-Rosen, F. Wigley, and A. Rosen (1997)
J. Exp. Med. 185, 71-80
   Abstract »    Full Text »    PDF »
Identification of a Nucleolin Binding Site in Human Topoisomerase I.
A. K. Bharti, M. O. J. Olson, D. W. Kufe, and E. H. Rubin (1996)
J. Biol. Chem. 271, 1993-1997
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CD34+ Spindle-Shaped Cells Selectively Disappear From the Skin Lesion of Scleroderma.
S. Aiba, N. Tabata, H. Ohtani, and H. Tagami (1994)
Arch Dermatol 130, 593-597
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Characterization and immunolocalization of RNA polymerase I transcription factor UBF with anti-NOR serum in protozoa, higher plant and vertebrate cells.
R. M. Rodrigo, M. C. Rendon, J. Torreblanca, G. Garcia-Herdugo, and F. J. Moreno (1992)
J. Cell Sci. 103, 1053-1063
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Whither the ANA?.
E. A. Lerner and M. R. Lerner (1987)
Arch Dermatol 123, 358-362
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