Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 31 January 1986:
Vol. 231. no. 4737, pp. 488 - 491
DOI: 10.1126/science.3079919
Prev | Table of Contents | Next

Articles

Science, Vol 231, Issue 4737, 488-491
Copyright © 1986 by American Association for the Advancement of Science

articles

Indirect observation by 13C NMR spectroscopy of a novel CO2 fixation pathway in methanogens

JN Evans, CJ Tolman, and MF Roberts

High-field carbon-13 nuclear magnetic resonance (NMR) spectroscopy has been used to monitor the isotopic dilution of doubly carbon-13-labeled precursors for 2,3-cyclopyrophosphoglycerate, a novel primary metabolite that occurs in certain methanogens. A unique carbon dioxide fixation pathway that gives rise to asymmetric labeling of acetyl coenzyme A has been demonstrated in Methanobacterium thermoautotrophicum. The effect of selected metabolic inhibitors on the labeled species in the pathway has been examined by NMR. These techniques establish a general, sensitive method for the delineation of convergent biosynthetic pathways.




To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)