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Science 20 December 1985:
Vol. 230. no. 4732, pp. 1378 - 1381
DOI: 10.1126/science.2999983
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Articles

Science, Vol 230, Issue 4732, 1378-1381
Copyright © 1985 by American Association for the Advancement of Science

articles

Promoter region of the human Harvey ras proto-oncogene: similarity to the EGF receptor proto-oncogene promoter

S Ishii, GT Merlino, and I Pastan

Regulation of transcription of members of the ras gene family undoubtably plays an important role in controlling cellular growth. Examination of this level of regulation requires identification of the promoter regions of the ras proto-oncogenes. Four major transcriptional start sites were detected in the human Harvey ras 1 proto-oncogene. The promoter region contains neither a TATA box nor a CAAT box in their characteristic upstream positions, has an extremely high G+C content (80 percent), and contains multiple GC boxes including seven CCGCCC repeats and three repeats of the inverted complement, GGGCGG. This region has strong promoter activity when placed upstream from the chloramphenicol acetyl transferase gene and transfected into monkey CV1 cells. In these ways the Harvey ras 1 proto-oncogene promoter resembles the promoter of the gene encoding the epidermal growth factor (EGF) receptor. The similarity between the two proto-oncogene promoters may be relevant to the mechanism by which the expression of such "growth control" genes is regulated.


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Binding of the Sp1 transcription factor by the human Harvey ras1 proto-oncogene promoter.
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