Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.

Science 20 December 1985:
Vol. 230. no. 4732, pp. 1350 - 1354
DOI: 10.1126/science.2999980
Prev | Table of Contents | Next

Articles

Science, Vol 230, Issue 4732, 1350-1354
Copyright © 1985 by American Association for the Advancement of Science

articles

Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia

RK Saiki, S Scharf, F Faloona, KB Mullis, GT Horn, HA Erlich, and N Arnheim

Two new methods were used to establish a rapid and highly sensitive prenatal diagnostic test for sickle cell anemia. The first involves the primer-mediated enzymatic amplification of specific beta-globin target sequences in genomic DNA, resulting in the exponential increase (220,000 times) of target DNA copies. In the second technique, the presence of the beta A and beta S alleles is determined by restriction endonuclease digestion of an end-labeled oligonucleotide probe hybridized in solution to the amplified beta-globin sequences. The beta-globin genotype can be determined in less than 1 day on samples containing significantly less than 1 microgram of genomic DNA.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Role of Activins and Inducible Nitric Oxide in the Pathogenesis of Ectopic Pregnancy in Patients with or without Chlamydia trachomatis Infection.
B. Refaat, M. Al-Azemi, I. Geary, A. Eley, and W. Ledger (2009)
Clin. Vaccine Immunol. 16, 1493-1503
   Abstract »    Full Text »    PDF »
Towards a new standard in slug species descriptions: the case of Limax sarnensis Heim & Nitz n. sp. (Pulmonata: Limacidae) from the Western Central Alps.
B. Nitz, R. Heim, U. E. Schneppat, I. Hyman, and G. Haszprunar (2009)
J. Mollus. Stud. 75, 279-294
   Abstract »    Full Text »    PDF »
Accuracy of Malaria Diagnosis by Microscopy, Rapid Diagnostic Test, and PCR Methods and Evidence of Antimalarial Overprescription in Non-Severe Febrile Patients in Two Tanzanian Hospitals.
E. Nicastri, N. Bevilacqua, M. S. Schepisi, M. G. Paglia, S. Meschi, S. M. Ame, J. A. Mohamed, S. Mangi, R. Fumakule, A. Di Caro, et al. (2009)
Am J Trop Med Hyg 80, 712-717
   Abstract »    Full Text »    PDF »
Sensitive isothermal detection of nucleic-acid sequence by primer generation-rolling circle amplification.
T. Murakami, J. Sumaoka, and M. Komiyama (2009)
Nucleic Acids Res. 37, e19
   Abstract »    Full Text »    PDF »
Classification of lymphoid neoplasms: the microscope as a tool for disease discovery.
E. S. Jaffe, N. L. Harris, H. Stein, and P. G. Isaacson (2008)
Blood 112, 4384-4399
   Abstract »    Full Text »    PDF »
Specificity of L1 Peptides versus Virus-Like Particles for Detection of Human Papillomavirus-Positive Cervical Lesions in Females Attending Engativa Hospital, Bogota, Colombia.
M. Urquiza, R. Sanchez, J. Amaya, S. Leon, J. Acosta, M. A. Patarroyo, M. Camargo, and M. E. Patarroyo (2008)
J. Clin. Microbiol. 46, 3714-3720
   Abstract »    Full Text »    PDF »
The Asexual Pathogen Aspergillus fumigatus Expresses Functional Determinants of Aspergillus nidulans Sexual Development.
V. Grosse and S. Krappmann (2008)
Eukaryot. Cell 7, 1724-1732
   Abstract »    Full Text »    PDF »
A Q312X mutation in the hemojuvelin gene is associated with cardiomyopathy due to juvenile haemochromatosis.
Y. Nagayoshi, M. Nakayama, S. Suzuki, J. Hokamaki, H. Shimomura, K. Tsujita, M. Fukuda, T. Yamashita, Y. Nakamura, S. Sugiyama, et al. (2008)
Eur J Heart Fail 10, 1001-1006
   Abstract »    Full Text »    PDF »
Melon Chlorotic Leaf Curl Virus: Characterization and Differential Reassortment with Closest Relatives Reveal Adaptive Virulence in the Squash Leaf Curl Virus Clade and Host Shifting by the Host-Restricted Bean Calico Mosaic Virus.
A. M. Idris, K. Mills-Lujan, K. Martin, and J. K. Brown (2008)
J. Virol. 82, 1959-1967
   Abstract »    Full Text »    PDF »
A Real-Time Polymerase Chain Reaction Assay for the Identification and Quantification of American Leishmania Species on the Basis of Glucose-6-Phosphate Dehydrogenase.
T. M. Castilho, L. M. A. Camargo, D. McMahon-Pratt, J. J. Shaw, and L. M. Floeter-Winter (2008)
Am J Trop Med Hyg 78, 122-132
   Abstract »    Full Text »    PDF »
Interspecies Interactions within Oral Microbial Communities.
H. K. Kuramitsu, X. He, R. Lux, M. H. Anderson, and W. Shi (2007)
Microbiol. Mol. Biol. Rev. 71, 653-670
   Abstract »    Full Text »    PDF »
Characterization of bacterial operons consisting of two tubulins and a kinesin-like gene by the novel Two-Step Gene Walking method.
M. Pilhofer, A. P. Bauer, M. Schrallhammer, L. Richter, W. Ludwig, K.-H. Schleifer, and G. Petroni (2007)
Nucleic Acids Res. 35, e135
   Abstract »    Full Text »    PDF »
Sequence specific detection of DNA using nicking endonuclease signal amplification (NESA).
T. Kiesling, K. Cox, E. A. Davidson, K. Dretchen, G. Grater, S. Hibbard, R. S. Lasken, J. Leshin, E. Skowronski, and M. Danielsen (2007)
Nucleic Acids Res. 35, e117
   Abstract »    Full Text »    PDF »
Highly Efficient Isothermal DNA Amplification System Using Three Elements of 5'-DNA-RNA-3' Chimeric Primers, RNaseH and Strand-displacing DNA Polymerase.
H. Mukai, T. Uemori, O. Takeda, E. Kobayashi, J. Yamamoto, K. Nishiwaki, T. Enoki, H. Sagawa, K. Asada, and I. Kato (2007)
J. Biochem. 142, 273-281
   Abstract »    Full Text »    PDF »
Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends.
C. Zhang and D. Xing (2007)
Nucleic Acids Res. 35, 4223-4237
   Abstract »    Full Text »    PDF »
Characterization of two novel cutaneous human papillomaviruses, HPV93 and HPV96.
N. Vasiljevic, K. Hazard, L. Eliasson, H. Ly, A. Hunziker, E.-M. de Villiers, B. Norrild, J. Dillner, and O. Forslund (2007)
J. Gen. Virol. 88, 1479-1483
   Abstract »    Full Text »    PDF »
Real-time PCR Assay for Ultrasensitive Quantification of DNA-Binding Proteins.
P. Hou, Z. Chen, M. Ji, N. He, and Z. Lu (2007)
Clin. Chem. 53, 581-586
   Abstract »    Full Text »    PDF »
Role of the monoclonal {kappa} chain V domain and reversibility of renal damage in a transgenic model of acquired Fanconi syndrome.
C. Sirac, F. Bridoux, C. Carrion, O. Devuyst, B. Fernandez, J.-M. Goujon, C. E. Hamel, J.-C. Aldigier, G. Touchard, and M. Cogne (2006)
Blood 108, 536-543
   Abstract »    Full Text »    PDF »
Ultra fast miniaturized real-time PCR: 40 cycles in less than six minutes.
P. Neuzil, C. Zhang, J. Pipper, S. Oh, and L. Zhuo (2006)
Nucleic Acids Res. 34, e77
   Abstract »    Full Text »    PDF »
No evidence of parvovirus B19 in tissue samples from patients with polyarteritis nodosa and microscopic polyangiitis as assessed by the polymerase chain reaction.
Z Sachetto, S C B Costa, P D Andrade, R A Conde, E M I Amstalden, A M Samara, and S R M Fernandes (2006)
Ann Rheum Dis 65, 418-420
   Full Text »    PDF »
Amplicon DNA Melting Analysis for Mutation Scanning and Genotyping: Cross-Platform Comparison of Instruments and Dyes.
M. G. Herrmann, J. D. Durtschi, L. K. Bromley, C. T. Wittwer, and K. V. Voelkerding (2006)
Clin. Chem. 52, 494-503
   Abstract »    Full Text »    PDF »
The polymerase chain reaction patents: going, going,... still going.
D. McDowell (2006)
J R Soc Med 99, 62-64
   Full Text »    PDF »
Multiplexed Detection of Anthrax-Related Toxin Genes.
M. J. Moser, D. R. Christensen, D. Norwood, and J. R. Prudent (2006)
J. Mol. Diagn. 8, 89-96
   Abstract »    Full Text »    PDF »
Absence of Kaposi's Sarcoma-associated Herpesvirus in Patients with Pulmonary Arterial Hypertension.
C. Henke-Gendo, M. Mengel, M. M. Hoeper, K. Alkharsah, and T. F. Schulz (2005)
Am. J. Respir. Crit. Care Med. 172, 1581-1585
   Abstract »    Full Text »    PDF »
Multiplexed tandem PCR: gene profiling from small amounts of RNA using SYBR Green detection.
K. K. Stanley and E. Szewczuk (2005)
Nucleic Acids Res. 33, e180
   Abstract »    Full Text »    PDF »
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis.
J. A. Jordan and M. B. Durso (2005)
J. Mol. Diagn. 7, 575-581
   Abstract »    Full Text »    PDF »
Hemophilia: A Practical Approach to Genetic Testing.
R. K. Pruthi (2005)
Mayo Clin. Proc. 80, 1485-1499
   Abstract »    PDF »
Proximity ligation assays with peptide conjugate 'burrs' for the sensitive detection of spores.
S. Pai, A. D. Ellington, and M. Levy (2005)
Nucleic Acids Res. 33, e162
   Abstract »    Full Text »    PDF »
Exo-Taq-Based Detection of DNA-Binding Protein for Homogeneous and Microarray Format.
T. Fukumori, H. Miyachi, and K. Yokoyama (2005)
J. Biochem. 138, 473-478
   Abstract »    Full Text »    PDF »
The power of real-time PCR.
M. A. Valasek and J. J. Repa (2005)
Advan Physiol Educ 29, 151-159
   Abstract »    Full Text »    PDF »
CHLAMYDIA TRACHOMATIS AND GENITAL HUMAN PAPILLOMAVIRUS INFECTIONS IN FEMALE UNIVERSITY STUDENTS IN HONDURAS.
N. TABORA, A. ZELAYA, J. BAKKERS, W. J. G. MELCHERS, and A. FERRERA (2005)
Am J Trop Med Hyg 73, 50-53
   Abstract »    Full Text »    PDF »
DNA as Evidence -- The Technology of Identification.
P. Gill (2005)
N. Engl. J. Med. 352, 2669-2671
   Full Text »    PDF »
Oligonucleotide-assisted cleavage and ligation: a novel directional DNA cloning technology to capture cDNAs. Application in the construction of a human immune antibody phage-display library.
S. Schoonbroodt, N. Frans, M. DeSouza, R. Eren, S. Priel, N. Brosh, J. Ben-Porath, A. Zauberman, E. Ilan, S. Dagan, et al. (2005)
Nucleic Acids Res. 33, e81
   Abstract »    Full Text »    PDF »
Development and Use of an Internal Positive Control for Detection of Bordetella pertussis by PCR.
S. Herwegh, C. Carnoy, F. Wallet, C. Loiez, and R. J. Courcol (2005)
J. Clin. Microbiol. 43, 2462-2464
   Abstract »    Full Text »    PDF »
Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments.
F. Dahl, M. Gullberg, J. Stenberg, U. Landegren, and M. Nilsson (2005)
Nucleic Acids Res. 33, e71
   Abstract »    Full Text »    PDF »
Expression of Interferon-inducible RNA Adenosine Deaminase ADAR1 during Pathogen Infection and Mouse Embryo Development Involves Tissue-selective Promoter Utilization and Alternative Splicing.
C. X. George, M. V. Wagner, and C. E. Samuel (2005)
J. Biol. Chem. 280, 15020-15028
   Abstract »    Full Text »    PDF »
Presence of DNA of Human Papillomavirus 16 but No Other Types in Tumor-Free Tonsillar Tissue.
R. Chen, P. Sehr, T. Waterboer, I. Leivo, M. Pawlita, A. Vaheri, and L.-M. Aaltonen (2005)
J. Clin. Microbiol. 43, 1408-1410
   Abstract »    Full Text »    PDF »
A genotyping system capable of simultaneously analyzing >1000 single nucleotide polymorphisms in a haploid genome.
H.-Y. Wang, M. Luo, I. V. Tereshchenko, D. M. Frikker, X. Cui, J. Y. Li, G. Hu, Y. Chu, M. A. Azaro, Y. Lin, et al. (2005)
Genome Res. 15, 276-283
   Abstract »    Full Text »    PDF »
Antiviral Effects of Human Immunodeficiency Virus Type 1-Specific Small Interfering RNAs against Targets Conserved in Select Neurotropic Viral Strains.
R. S. Dave and R. J. Pomerantz (2004)
J. Virol. 78, 13687-13696
   Abstract »    Full Text »    PDF »
Phenylphosphate Synthase: a New Phosphotransferase Catalyzing the First Step in Anaerobic Phenol Metabolism in Thauera aromatica.
S. Schmeling, A. Narmandakh, O. Schmitt, N. Gad'on, K. Schuhle, and G. Fuchs (2004)
J. Bacteriol. 186, 8044-8057
   Abstract »    Full Text »    PDF »
Xin repeats define a novel actin-binding motif.
D. Pacholsky, P. Vakeel, M. Himmel, T. Lowe, T. Stradal, K. Rottner, D. O. Furst, and P. F. M. van der Ven (2004)
J. Cell Sci. 117, 5257-5268
   Abstract »    Full Text »    PDF »
Diagnosis and Assessment of Trachoma.
A. W. Solomon, R. W. Peeling, A. Foster, and D. C. W. Mabey (2004)
Clin. Microbiol. Rev. 17, 982-1011
   Abstract »    Full Text »    PDF »
Analysis of the medium (M) segment sequence of Guaroa virus and its comparison to other orthobunyaviruses.
T. Briese, A. Rambaut, and W. I. Lipkin (2004)
J. Gen. Virol. 85, 3071-3077
   Abstract »    Full Text »    PDF »
Academia-Industry Collaboration: An Integral Element for Building "Omic" Resources.
D. E. Hill, M. A. Brasch, A. A. del Campo, L. Doucette-Stamm, J. I. Garrels, J. Glaven, J. L. Hartley, J. R. Hudson Jr., T. Moore, and M. Vidal (2004)
Genome Res. 14, 2010-2014
   Full Text »    PDF »
Extraction and Amplification of Genomic DNA from Human Blood on Nanoporous Aluminum Oxide Membranes.
M. G. Elgort, M. G. Herrmann, M. Erali, J. D. Durtschi, K. V. Voelkerding, and R. E. Smith (2004)
Clin. Chem. 50, 1817-1819
   Full Text »    PDF »
Molecular barcodes detect redundancy and contamination in hairpin-bisulfite PCR.
B. E. Miner, R. J. Stoger, A. F. Burden, C. D. Laird, and R. S. Hansen (2004)
Nucleic Acids Res. 32, e135
   Abstract »    Full Text »    PDF »
Morules in endometrial carcinoma and benign endometrial lesions differ from squamous differentiation tissue and are not infected with human papillomavirus.
K Chinen, K Kamiyama, T Kinjo, A Arasaki, Y Ihama, T Hamada, and T Iwamasa (2004)
J. Clin. Pathol. 57, 918-926
   Abstract »    Full Text »    PDF »
The Homogentisate Pathway: a Central Catabolic Pathway Involved in the Degradation of L-Phenylalanine, L-Tyrosine, and 3-Hydroxyphenylacetate in Pseudomonas putida.
E. Arias-Barrau, E. R. Olivera, J. M. Luengo, C. Fernandez, B. Galan, J. L. Garcia, E. Diaz, and B. Minambres (2004)
J. Bacteriol. 186, 5062-5077
   Abstract »    Full Text »    PDF »
Human Chromosome 21-Specific DNA Markers Are Useful in Prenatal Detection of Down Syndrome.
J.-D. Liou, D.-C. Chu, P.-J. Cheng, S.-D. Chang, C.-F. Sun, Y.-C. Wu, W.-Y. Liou, and D.T.Y. Chiu (2004)
Ann. Clin. Lab. Sci. 34, 319-323
   Abstract »    Full Text »    PDF »
Prospective Study of a Real-Time PCR That Is Highly Sensitive, Specific, and Clinically Useful for Diagnosis of Meningococcal Disease in Children.
P. A. Bryant, H. Y. Li, A. Zaia, J. Griffith, G. Hogg, N. Curtis, and J. R. Carapetis (2004)
J. Clin. Microbiol. 42, 2919-2925
   Abstract »    Full Text »    PDF »
A Miniaturized Column Chromatography Method for Measuring Receptor-Mediated Inositol Phosphate Accumulation.
E. R. Benjamin, S. L. Haftl, D. N. Xanthos, G. Crumley, M. Hachicha, and K. J. Valenzano (2004)
J Biomol Screen 9, 343-353
   Abstract »    PDF »
Phylogenetic Relationships among Virulent Newcastle Disease Virus Isolates from the 2002-2003 Outbreak in California and Other Recent Outbreaks in North America.
J. C. Pedersen, D. A. Senne, P. R. Woolcock, H. Kinde, D. J. King, M. G. Wise, B. Panigrahy, and B. S. Seal (2004)
J. Clin. Microbiol. 42, 2329-2334
   Abstract »    Full Text »    PDF »
Novel Internal Controls For Real-Time PCR Assays.
F. S. Nolte (2004)
Clin. Chem. 50, 801-802
   Full Text »    PDF »
Human herpesvirus 8 (HHV8) sequence variations in HHV8 related tumours in Okinawa, a subtropical island in southern Japan.
K Kamiyama, T Kinjo, K Chinen, T Iwamasa, H Uezato, J-i Miyagi, N Mori, and N Yamane (2004)
J. Clin. Pathol. 57, 529-535
   Abstract »    Full Text »    PDF »
Evolutionary Strata on the Mouse X Chromosome Correspond to Strata on the Human X Chromosome.
S. A. Sandstedt and P. K. Tucker (2004)
Genome Res. 14, 267-272
   Abstract »    Full Text »    PDF »
Semen Quality of Men With Asymptomatic Chlamydial Infection.
S. Hosseinzadeh, A. Eley, and A. A. Pacey (2004)
J Androl 25, 104-109
   Abstract »    Full Text »    PDF »
Neurogenetics: single gene disorders.
S-M Pulst (2003)
J. Neurol. Neurosurg. Psychiatry 74, 1608-1614
   Abstract »    Full Text »    PDF »
Nanoparticle-Based Bio-Bar Codes for the Ultrasensitive Detection of Proteins.
J.-M. Nam, C. S. Thaxton, and C. A. Mirkin (2003)
Science 301, 1884-1886
   Abstract »    Full Text »    PDF »
Coordinate Regulation of the Escherichia coli Formate Dehydrogenase fdnGHI and fdhF Genes in Response to Nitrate, Nitrite, and Formate: Roles for NarL and NarP.
H. Wang and R. P. Gunsalus (2003)
J. Bacteriol. 185, 5076-5085
   Abstract »    Full Text »    PDF »
Evaluation of the Use of PCR and Reverse Transcriptase PCR for Detection of Pathogenic Bacteria in Biosolids from Anaerobic Digestors and Aerobic Composters.
C. Burtscher and S. Wuertz (2003)
Appl. Envir. Microbiol. 69, 4618-4627
   Abstract »    Full Text »    PDF »
Comparison of Two Quantitative Polymerase Chain Reaction Methods for Detecting HER2/neu Amplification.
A. Millson, A. Suli, L. Hartung, S. Kunitake, A. Bennett, M. C. L. Nordberg, W. Hanna, C. T. Wittwer, A. Seth, and E. Lyon (2003)
J. Mol. Diagn. 5, 184-190
   Abstract »    Full Text »    PDF »
Presence of Viral Genomes in Mineral Water: a Sufficient Condition To Assume Infectious Risk?.
B. Gassilloud, L. Schwartzbrod, and C. Gantzer (2003)
Appl. Envir. Microbiol. 69, 3965-3969
   Abstract »    Full Text »    PDF »
The minisequencing method: an alternative strategy for preimplantation genetic diagnosis of single gene disorders.
F. Fiorentino, M.C. Magli, D. Podini, A.P. Ferraretti, A. Nuccitelli, N. Vitale, M. Baldi, and L. Gianaroli (2003)
Mol. Hum. Reprod. 9, 399-410
   Abstract »    Full Text »    PDF »
Asymptomatic carriage of Pneumocystis jiroveci in subjects undergoing bronchoscopy: a prospective study.
N A Maskell, D J Waine, A Lindley, J C T Pepperell, A E Wakefield, R F Miller, and R J O Davies (2003)
Thorax 58, 594-597
   Abstract »    Full Text »    PDF »
Varicella-Zoster Virus DNA in Cells Isolated from Human Trigeminal Ganglia.
M. J. Levin, G.-Y. Cai, M. D. Manchak, and L. I. Pizer (2003)
J. Virol. 77, 6979-6987
   Abstract »    Full Text »    PDF »
Comparison of Washing and Swabbing Procedures for Collecting Genital Fluids To Assess Cervicovaginal Shedding of Herpes Simplex Virus Type 2 DNA.
A. Ndjoyi-Mbiguino, F. Ozouaki, J. Legoff, F.-X. Mbopi-Keou, A. Si-Mohamed, I. N. Onas, E. Avoune, and L. Belec (2003)
J. Clin. Microbiol. 41, 2662-2664
   Abstract »    Full Text »    PDF »
A novel multiplex quantitative DNA array based PCR (MQDA-PCR) for quantification of transgenic maize in food and feed.
K. Rudi, I. Rud, and A. Holck (2003)
Nucleic Acids Res. 31, e62
   Abstract »    Full Text »    PDF »
COMPARISON OF A POLYMERASE CHAIN REACTION AND THE KATO-KATZ TECHNIQUE FOR DIAGNOSING INFECTION WITH SCHISTOSOMA MANSONI.
L. A. PONTES, M. C. OLIVEIRA, N. KATZ, E. DIAS-NETO, and A. RABELLO (2003)
Am J Trop Med Hyg 68, 652-656
   Abstract »    Full Text »    PDF »
CHRONIC CHAGAS' DISEASE IN RHESUS MONKEYS (MACACA MULATTA): EVALUATION OF PARASITEMIA, SEROLOGY, ELECTROCARDIOGRAPHY, ECHOCARDIOGRAPHY, AND RADIOLOGY.
C. M. E. CARVALHO, M. C. R. ANDRADE, S. S. XAVIER, R. H. R. MANGIA, C. C. BRITTO, A. M. JANSEN, O. FERNANDES, J. LANNES-VIEIRA, and M. D. G. BONECINI-ALMEIDA (2003)
Am J Trop Med Hyg 68, 683-691
   Abstract »    Full Text »    PDF »
Prospective study on cervical neoplasia: presence of HPV DNA in cytological smears precedes the development of cervical neoplastic lesions.
R Tachezy, M Salakova, E Hamsikova, J Kanka, A Havrankova, and V Vonka (2003)
Sex Transm Inf 79, 191-196
   Abstract »    Full Text »    PDF »
The Role of Apoptosis Signal-regulating Kinase 1 in Lymphotoxin-beta Receptor-mediated Cell Death.
M.-C. Chen, M.-J. Hwang, Y.-C. Chou, W.-H. Chen, G. Cheng, H. Nakano, T.-Y. Luh, S.-C. Mai, and S.-L. Hsieh (2003)
J. Biol. Chem. 278, 16073-16081
   Abstract »    Full Text »    PDF »
Mutations Which Uncouple Transport and Phosphorylation in the D-Mannitol Phosphotransferase System of Escherichia coli K-12 and Klebsiella pneumoniae 1033-5P14.
S. Otte, A. Scholle, S. Turgut, and J. W. Lengeler (2003)
J. Bacteriol. 185, 2267-2276
   Abstract »    Full Text »    PDF »
Effect of Amino Acid Substitution of the V3 and Bridging Sheet Residues in Human Immunodeficiency Virus Type 1 Subtype C gp120 on CCR5 Utilization.
P. Suphaphiphat, A. Thitithanyanont, S. Paca-Uccaralertkun, M. Essex, and T.-H. Lee (2003)
J. Virol. 77, 3832-3837
   Abstract »    Full Text »    PDF »
Pathogenic Human Viruses in Coastal Waters.
D. W. Griffin, K. A. Donaldson, J. H. Paul, and J. B. Rose (2003)
Clin. Microbiol. Rev. 16, 129-143
   Abstract »    Full Text »    PDF »
Molecular analysis of Tripterygium hypoglaucum (level) Hutch-induced mutations at the HPRT locus in human promyelocytic leukemia cells by multiplex polymerase chain reaction.
S. X. Liu, J. Cao, and H. An (2003)
Mutagenesis 18, 77-80
   Abstract »    Full Text »    PDF »
Conflict between nuclear and mitochondrial DNA phylogenies of a recent species radiation: What mtDNA reveals and conceals about modes of speciation in Hawaiian crickets.
K. L. Shaw (2002)
PNAS 99, 16122-16127
   Abstract »    Full Text »    PDF »
MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.
A. Yamane (2002)
Nucleic Acids Res. 30, e97
   Abstract »    Full Text »    PDF »
Causes of Common Illnesses: An Overview.
T. Babinchak (2002)
The Journal of School Nursing 18, 8-11
   Full Text »    PDF »
The Science Behind Lysol: Relevance for Schools.
J. R. Rubino and D. Gaber (2002)
The Journal of School Nursing 18, 15-18
   Full Text »    PDF »
Samarangenin B from Limonium sinense Suppresses Herpes Simplex Virus Type 1 Replication in Vero Cells by Regulation of Viral Macromolecular Synthesis.
Y.-C. Kuo, L.-C. Lin, W.-J. Tsai, C.-J. Chou, S.-H. Kung, and Y.-H. Ho (2002)
Antimicrob. Agents Chemother. 46, 2854-2864
   Abstract »    Full Text »    PDF »
A Relationship Between Varicella-Zoster Virus-Specific Delayed Hypersensitivity and Varicella-Zoster Virus-Induced Anterior Uveitis.
T. Kezuka, J.-i. Sakai, H. Minoda, M. Takeuchi, H. Keino, J. W. Streilein, and M. Usui (2002)
Arch Ophthalmol 120, 1183-1188
   Abstract »    Full Text »    PDF »
Primer on Medical Genomics Part II: Background Principles and Methods in Molecular Genetics.
A. Tefferi, E. D. Wieben, G. W. Dewald, D. A. H. Whiteman, M. E. Bernard, and T. C. Spelsberg (2002)
Mayo Clin. Proc. 77, 785-808
   Abstract »    PDF »
Multiple label-free biodetection and quantitative DNA-binding assays on a nanomechanical cantilever array.
R. McKendry, J. Zhang, Y. Arntz, T. Strunz, M. Hegner, H. P. Lang, M. K. Baller, U. Certa, E. Meyer, H.-J. Guntherodt, et al. (2002)
PNAS 99, 9783-9788
   Abstract »    Full Text »    PDF »
Medulloblastomas and primitive neuroectodermal tumors rarely contain polyomavirus DNA sequences.
J. Y.H. Kim, I. J. Koralnik, M. LeFave, R. A. Segal, L.-A. Pfister, and S. L. Pomeroy (2002)
Neuro-oncol 4, 165-170
   Abstract »    PDF »
Functional analysis of the Saccharomyces cerevisiae DUP240 multigene family reveals membrane-associated proteins that are not essential for cell viability.
R. Poirey, L. Despons, V. Leh, M.-J. Lafuente, S. Potier, J.-L. Souciet, and J.-C. Jauniaux (2002)
Microbiology 148, 2111-2123
   Abstract »    Full Text »    PDF »
Epstein-Barr virus (EBV) subtype in EBV related oral squamous cell carcinoma in Okinawa, a subtropical island in southern Japan, compared with Kitakyushu and Kumamoto in mainland Japan.
M Higa, T Kinjo, K Kamiyama, T Iwamasa, T Hamada, and K Iyama (2002)
J. Clin. Pathol. 55, 414-423
   Abstract »    Full Text »    PDF »
Renin-angiotensin system expression and secretory function in cultured human ciliary body non-pigmented epithelium.
A B Cullinane, P S Leung, J Ortego, M Coca-Prados, and B J Harvey (2002)
Br J Ophthalmol 86, 676-683
   Abstract »    Full Text »    PDF »
A Luminescent Escherichia coli Biosensor for the High Throughput Detection of {beta}-Lactams.
S. J. Valtonen, J. S. Kurittu, and M. T. Karp (2002)
J Biomol Screen 7, 127-134
   Abstract »    PDF »
Muscle-specific RING finger-1 interacts with titin to regulate sarcomeric M-line and thick filament structure and may have nuclear functions via its interaction with glucocorticoid modulatory element binding protein-1.
A. S. McElhinny, K. Kakinuma, H. Sorimachi, S. Labeit, and C. C. Gregorio (2002)
J. Cell Biol. 157, 125-136
   Abstract »    Full Text »    PDF »
Prevalence of Parvovirus B19 and Parvovirus V9 DNA and Antibodies in Paired Bone Marrow and Serum Samples from Healthy Individuals.
E. D. Heegaard, B. L. Petersen, C. J. Heilmann, and A. Hornsleth (2002)
J. Clin. Microbiol. 40, 933-936
   Abstract »    Full Text »    PDF »
Active versus borderline myocarditis: clinicopathological correlates and prognostic implications.
A Angelini, M Crosato, G M Boffa, F Calabrese, V Calzolari, R Chioin, L Daliento, and G Thiene (2002)
Heart 87, 210-215
   Abstract »    Full Text »    PDF »
Acyclic and dideoxy terminator preferences denote divergent sugar recognition by archaeon and Taq DNA polymerases.
A. F. Gardner and W. E. Jack (2002)
Nucleic Acids Res. 30, 605-613
   Abstract »    Full Text »    PDF »
Preimplantation genetic diagnosis in clinical practice.
E Kanavakis and J Traeger-Synodinos (2002)
J. Med. Genet. 39, 6-11
   Abstract »    Full Text »    PDF »
TaqMan-Based Detection of Trichomonas vaginalis DNA from Female Genital Specimens.
J. A. Jordan, D. Lowery, and M. Trucco (2001)
J. Clin. Microbiol. 39, 3819-3822
   Abstract »    Full Text »    PDF »
Characterization of Mutations in the metY-nusA-infB Operon That Suppress the Slow Growth of a {Delta}rimM Mutant.
G. O. Bylund, J. M. Lovgren, and P. M. Wikstrom (2001)
J. Bacteriol. 183, 6095-6106
   Abstract »    Full Text »    PDF »


To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)