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Science 20 December 1985:
Vol. 230. no. 4732, pp. 1344 - 1349
DOI: 10.1126/science.2933810
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Articles

Science, Vol 230, Issue 4732, 1344-1349
Copyright © 1985 by American Association for the Advancement of Science

articles

The 3' splice site of pre-messenger RNA is recognized by a small nuclear ribonucleoprotein

B Chabot, DL Black, DM LeMaster, and JA Steitz

A component present in splicing extracts selectively binds the 3' splice site of a precursor messenger RNA (pre-mRNA) transcript of a human beta-globin gene. Since this component can be immunoprecipitated by either autoantibodies of the Sm class or antibodies specifically directed against trimethylguanosine, it is a small nuclear ribonucleoprotein (snRNP). Its interaction with the 3' splice site occurs rapidly even at 0 degrees C, does not require adenosine triphosphate, and is altered by certain mutations in the 3' splice site region. Binding is surprisingly insensitive to treatment of the extract with micrococcal nuclease. The U5 particle is the only abundant Sm snRNP with a capped 5' end that is equally resistant to micrococcal nuclease. This suggests that, in addition to the U1 and U2 snRNP's, U5 snRNP's participate in pre-mRNA splicing.


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