Note to users. If you're seeing this message, it means that your browser cannot find this page's style/presentation instructions -- or possibly that you are using a browser that does not support current Web standards. Find out more about why this message is appearing, and what you can do to make your experience of our site the best it can be.
Science Policy Alerts

Site Tools

  • AAAS
  • Subscribe
  • Feedback

Site Search

Search Advanced

Science 13 December 1985:
Vol. 230. no. 4731, pp. 1282 - 1285
DOI: 10.1126/science.2999978
Prev | Table of Contents | Next

Articles

Science, Vol 230, Issue 4731, 1282-1285
Copyright © 1985 by American Association for the Advancement of Science

articles

The human gene encoding GM-CSF is at 5q21-q32, the chromosome region deleted in the 5q- anomaly

K Huebner, M Isobe, CM Croce, DW Golde, SE Kaufman, and JC Gasson

Human granulocyte-macrophage colony-stimulating factor (GM-CSF) is a 22,000-dalton glycoprotein that stimulates the growth of myeloid progenitor cells and acts directly on mature neutrophils. A full-length complementary DNA clone encoding human GM-CSF was used as a probe to screen a human genomic library and isolate the gene encoding human GM-CSF. The human GM-CSF gene is approximately 2.5 kilobase pairs in length with at least three intervening sequences. The GM-CSF gene was localized by somatic cell hybrid analysis and in situ hybridization to human chromosome region 5q21-5q32, which is involved in interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. An established, human promyelocytic leukemia cell line, HL60, contains a rearranged, partially deleted GM-CSF allele and a candidate 5q- marker chromosome, indicating that the truncated GM-CSF allele may reside at the rejoining point for the interstitial deletion on the HL60 marker chromosome.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
A contiguous high-resolution radiation hybrid map of 44 loci from the distal portion of the long arm of human chromosome 5..
J A Warrington and J J Wasmuth (1996)
Genome Res. 6, 628-632
   Abstract »    PDF »
Deletion of IRF-1, mapping to chromosome 5q31.1, in human leukemia and preleukemic myelodysplasia.
C. Willman, C. Sever, M. Pallavicini, H Harada, N Tanaka, M. Slovak, H Yamamoto, K Harada, T. Meeker, A. List, et al. (1993)
Science 259, 968-971
   Abstract »
The human hematopoietic colony-stimulating factors.
S. Clark and R Kamen (1987)
Science 236, 1229-1237
   Abstract »    PDF »
Evidence for the involvement of GM-CSF and FMS in the deletion (5q) in myeloid disorders.
M. Le Beau, C. Westbrook, M. Diaz, R. Larson, J. Rowley, J. Gasson, D. Golde, and C. Sherr (1986)
Science 231, 984-987
   Abstract »    PDF »


ADVERTISEMENT
Click Me!

To Advertise     Find Products

Science. ISSN 0036-8075 (print), 1095-9203 (online)