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Science 29 November 1985:
Vol. 230. no. 4729, pp. 1057 - 1061
DOI: 10.1126/science.3864246
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Articles

Science, Vol 230, Issue 4729, 1057-1061
Copyright © 1985 by American Association for the Advancement of Science

articles

Retroviral vector-mediated gene transfer into human hematopoietic progenitor cells

HE Gruber, KD Finley, RM Hershberg, SS Katzman, PK Laikind, JE Seegmiller, T Friedmann, JK Yee, and DJ Jolly

The transfer of the human gene for hypoxanthine phosphoribosyltransferase (HPRT) into human bone marrow cells was accomplished by use of a retroviral vector. The cells were infected in vitro with a replication-incompetent murine retroviral vector that carried and expressed a mutant HPRT complementary DNA. The infected cells were superinfected with a helper virus and maintained in long-term culture. The production of progeny HPRT virus by the bone marrow cells was demonstrated with a colony formation assay on cultured HPRT-deficient, ouabain-resistant murine fibroblasts. Hematopoietic progenitor cells able to form colonies of granulocytes or macrophages (or both) in semisolid medium in the presence of colony stimulating factor were present in the nonadherent cell population. Colony forming units cloned in agar and subsequently cultured in liquid medium produced progeny HPRT virus, indicating infection of this class of hematopoietic progenitor cell.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
Efficient retrovirus-mediated gene transfer to transplantable human bone marrow cells in the absence of fibronectin.
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An in vivo model of somatic cell gene therapy for human severe combined immunodeficiency.
G Ferrari, S Rossini, R Giavazzi, D Maggioni, N Nobili, M Soldati, G Ungers, F Mavilio, E Gilboa, and C Bordignon (1991)
Science 251, 1363-1366
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High-level recombinant gene expression in rabbit endothelial cells transduced by retroviral vectors.
J. Zwiebel, S. Freeman, P. Kantoff, K Cornetta, U. Ryan, and W. Anderson (1989)
Science 243, 220-222
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Gene Expression from a Transcriptionally Disabled Retroviral Vector.
J.-K. Yee, D.J. Jolly, J.C. Moores, J.G. Respess, and T. Friedmann (1986)
Cold Spring Harb Symp Quant Biol 51, 1021-1026
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Gene Transfer and Expression in Nonhuman Primates Using Retroviral Vectors.
W. F. Anderson, P. Kantoff, M. Eglitis, J. McLachlin, E. Karson, J. Zwiebel, A. Nienhuis, S. Karlsson, R.M. Blaese, D. Kohn, et al. (1986)
Cold Spring Harb Symp Quant Biol 51, 1073-1081
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