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Science 29 November 1985:
Vol. 230. no. 4729, pp. 1048 - 1051
DOI: 10.1126/science.2997930
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Articles
Science, Vol 230, Issue 4729, 1048-1051
Copyright © 1985 by American Association for the Advancement of Science
Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection
RD Schrier,
JA Nelson,
and
MB Oldstone
In situ hybridization was used to detect human cytomegalovirus (HCMV) in the peripheral blood mononuclear cells of some naturally infected (seropositive) individuals. A subpopulation of cells hybridized specifically to a portion of the HCMV genome that is heavily transcribed during the immediate-early period of infection. The hybridization signal was markedly reduced by base hydrolysis and ribonuclease, and therefore the probe appears to be detecting viral RNA. A fluorescence-activated cell sorter was used to select lymphocytes bearing the OKT4 and OKT8 markers. Hybridization with the HCMV probe revealed a higher proportion of positive cells in the OKT4 than in the OKT8 subset. This observation specifically identifies lymphocytes as a cell population involved in natural HCMV infection and suggests that lymphocytes may be a reservoir for maintaining infection and may also serve as a vehicle for its spread by blood transfusion.
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