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Science 29 November 1985:
Vol. 230. no. 4729, pp. 1003 - 1008
DOI: 10.1126/science.2865810
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Articles

Science, Vol 230, Issue 4729, 1003-1008
Copyright © 1985 by American Association for the Advancement of Science

articles

A glycophospholipid tail at the carboxyl terminus of the Thy-1 glycoprotein of neurons and thymocytes

AG Tse, AN Barclay, A Watts, and AF Williams

Cell surface molecules of eukaryotic cells have been considered to be integrated into the membrane bilayer by a transmembrane protein sequence. The Thy-1 antigen of rodent thymocytes and brain was the first eukaryotic membrane molecule for which biochemical data clearly suggested membrane integration via a nonprotein tail. Direct evidence is now presented showing that a glycophospholipid structure is attached to the carboxyl-terminal cysteine residue and that 31 carboxyl-terminal amino acids predicted from the Thy-1 complementary DNA sequence are not present in the mature glycoprotein. These experimental results raise questions concerning signaling across a cell membrane since antibodies to Thy-1 can stimulate T lymphocytes to release lymphokines and undergo cell division.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
The Extracellular Domain of the beta 1 Subunit Is Both Necessary and Sufficient for beta 1-like Modulation of Sodium Channel Gating.
K. A. McCormick, J. Srinivasan, K. White, T. Scheuer, and W. A. Catterall (1999)
J. Biol. Chem. 274, 32638-32646
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Peripheral CD4+ T Cell Maturation Recognized by Increased Expression of Thy-1/CD90 Bearing the 6C10 Carbohydrate Epitope.
M. Gui, D. L. Wiest, J. Li, D. Kappes, R. R. Hardy, and K. Hayakawa (1999)
J. Immunol. 163, 4796-4804
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Cleavage of the Glycosylphosphatidylinositol Anchor Affects the Reactivity of Thy-1 with Antibodies.
T. Kukulansky, S. Abramovitch, and N. Hollander (1999)
J. Immunol. 162, 5993-5997
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The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research.
M. Ferguson (1999)
J. Cell Sci. 112, 2799-2809
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Thy-1 Is a Component Common to Multiple Populations of Synaptic Vesicles.
C.-J. Jeng, S. A. McCarroll, T. F. J. Martin, E. Floor, J. Adams, D. Krantz, S. Butz, R. Edwards, and E. S. Schweitzer (1998)
J. Cell Biol. 140, 685-698
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Biosynthesis of the Side Chain of Yeast Glycosylphosphatidylinositol Anchors Is Operated by Novel Mannosyltransferases Located in the Endoplasmic Reticulum and the Golgi Apparatus.
G.ör. Sipos, A. Puoti, and A. Conzelmann (1995)
J. Biol. Chem. 270, 19709-19715
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Identification of subcellular compartments containing peptidylglycine alpha-amidating monooxygenase in rat anterior pituitary.
A. Oyarce and B. Eipper (1995)
J. Cell Sci. 108, 287-297
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Carboxyl terminus structural requirements for glycosyl-phosphatidylinositol anchor addition to cell surface proteins.
C Beghdadi-Rais, M Schreyer, M Rousseaux, P Borel, R. Eisenberg, G. Cohen, C Bron, and N Fasel (1993)
J. Cell Sci. 105, 831-840
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Efficient incorporation of human CD4 protein into avian leukosis virus particles.
J. Young, P Bates, K Willert, and H. Varmus (1990)
Science 250, 1421-1423
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An unusual form of lipid linkage to the CD45 peptide.
A Takeda and A. Maizel (1990)
Science 250, 676-679
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Gene targeting at the human CD4 locus by epitope addition..
M Jasin, S J Elledge, R W Davis, and P Berg (1990)
Genes & Dev. 4, 157-166
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Mechanism of membrane anchoring affects polarized expression of two proteins in MDCK cells.
D. Brown, B Crise, and J. Rose (1989)
Science 245, 1499-1501
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In vivo modulation of cytolytic activity and Thy-1 expression in TCR-gamma delta+ intraepithelial lymphocytes.
L Lefrancois and T Goodman (1989)
Science 243, 1716-1718
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Signal peptide for protein secretion directing glycophospholipid membrane anchor attachment.
I. Caras and G. Weddell (1989)
Science 243, 1196-1198
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Glycosyl-phosphatidylinositol moiety that anchors Trypanosoma brucei variant surface glycoprotein to the membrane.
M. Ferguson, S. Homans, R. Dwek, and T. Rademacher (1988)
Science 239, 753-759
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Structural and functional roles of glycosyl-phosphatidylinositol in membranes.
M. Low and A. Saltiel (1988)
Science 239, 268-275
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Signal for attachment of a phospholipid membrane anchor in decay accelerating factor.
I. Caras, G. Weddell, M. Davitz, V Nussenzweig, and D. Martin Jr (1987)
Science 238, 1280-1283
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A glycan-phosphatidylinositol-specific phospholipase D in human serum.
M. Davitz, D Hereld, S Shak, J Krakow, P. Englund, and V Nussenzweig (1987)
Science 238, 81-84
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