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Science 22 November 1985:
Vol. 230. no. 4728, pp. 943 - 945
DOI: 10.1126/science.3933111

Articles

Science, Vol 230, Issue 4728, 943-945
Copyright © 1985 by American Association for the Advancement of Science


articles

Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro

BJ Sugarman, BB Aggarwal, PE Hass, IS Figari, MA Palladino Jr, and HM Shepard

Modulation of the growth of human and murine cell lines in vitro by recombinant human tumor necrosis factor-alpha (rTNF-alpha) and recombinant human interferon-gamma (rIFN-gamma) was investigated. rTNF-alpha had cytostatic or cytolytic effects on only some tumor cell lines. When administered together with rIFN-gamma, rTNF-alpha showed enhanced antiproliferative effects on a subset of the cell lines tested. In contrast to its effects on sensitive tumor cells, rTNF-alpha augmented the growth of normal diploid fibroblasts. Variations in the proliferative response induced by rTNF-alpha were apparently not due to differences in either the number of binding sites per cell or their affinity for rTNF-alpha. These observations indicate that the effects of rTNF-alpha on cell growth are not limited to tumor cells, but rather that this protein may have a broad spectrum of activities in vivo.


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Interleukin-4 and -13 Inhibit Tumor Necrosis Factor-alpha mRNA Translational Activation in Lipopolysaccharide-induced Mouse Macrophages.
T. Mijatovic, V. Kruys, D. Caput, P. Defrance, and G. Huez (1997)
J. Biol. Chem. 272, 14394-14398
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Increased release of tumour necrosis factor-alpha in human tear fluid after excimer laser induced corneal wound.
M. Vesaluoma, A.-M. Teppo, C. Gronhagen-Riska, and T. Tervo (1997)
Br J Ophthalmol 81, 145-149
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Inducible Activation of RelB in Fibroblasts.
N. E. Olashaw and N. E. Olashaw (1996)
J. Biol. Chem. 271, 30307-30310
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Endothelial Cell Death Induced by Tumor Necrosis Factor-alpha Is Inhibited by the Bcl-2 Family Member, A1.
A. Karsan, E. Yee, and J. M. Harlan (1996)
J. Biol. Chem. 271, 27201-27204
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Myxoma Virus T2 Protein, a Tumor Necrosis Factor (TNF) Receptor Homolog, Is Secreted as a Monomer and Dimer That Each Bind Rabbit TNFalpha , but the Dimer Is a More Potent TNF Inhibitor.
M. Schreiber, K. Rajarathnam, and G. McFadden (1996)
J. Biol. Chem. 271, 13333-13341
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Apoptotic Inhibition of Head and Neck Squamous Cell Carcinoma Cells by Tumor Necrosis Factor {alpha}.
K. B. Briskin, C. Fady, M. Wang, and A. Lichtenstein (1996)
Arch Otolaryngol Head Neck Surg 122, 559-563
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Plasminogen Activator Inhibitor Type 2 Inhibits Tumor Necrosis Factor alpha-induced Apoptosis.
J. L. Dickinson, E. J. Bates, A. Ferrante, and T. M. Antalis (1995)
J. Biol. Chem. 270, 27894-27904
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Effects of Tumor Necrosis Factor-alpha on Antimitogenicity and Cell Cycle-related Proteins in MCF-7 Cells.
D.-i. Jeoung, B. Tang, and M. Sonenberg (1995)
J. Biol. Chem. 270, 18367-18373
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Involvement of Reactive Oxygen Species in Cytokine and Growth Factor Induction of c-fos Expression in Chondrocytes.
Y. Y. C. Lo and T. F. Cruz (1995)
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Tumor Necrosis Factor [IMAGE] Facilitates Nuclear Actions of Retinoic Acid to Regulate Expression of the Alkaline Phosphatase Gene in Preosteoblasts.
S. S. Manji, H. Zhou, D. M. Findlay, T. J. Martin, and K. W. Ng (1995)
J. Biol. Chem. 270, 8958-8962
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Enhanced Cytokine Production and Collagen Synthesis of Gingival Fibroblasts from Patients with Denture Fibromatosis.
K. Nakao, K. Yoneda, and T. Osaki (1995)
Journal of Dental Research 74, 1072-1078
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Cellular Proliferation and Activation of NFkappaB Are Induced by Autocrine Production of Tumor Necrosis Factor alpha in the Human T Lymphoma Line HuT 78.
M. A. O'Connell, R. Cleere, A. Long, and D. Kelleher (1995)
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Abnormal development of peripheral lymphoid organs in mice deficient in lymphotoxin.
P De Togni, J Goellner, N. Ruddle, P. Streeter, A Fick, S Mariathasan, S. Smith, R Carlson, L. Shornick, J Strauss-Schoenberger, et al. (1994)
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Liposomal Applications to Cancer Therapy.
Y. Namba and N. Oku (1993)
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Tumor Necrosis Factor and Chemotherapeutic Agents: Potentiation of Cytotoxicity With Interferon Gamma.
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Successful Combination Immunotherapy for the Generation In Vivo of Antitumor Activity With Anti-CD3, Interleukin 2, and Tumor Necrosis Factor {alpha}.
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Arch Surg 125, 220-225
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Are the Catabolic Effects of Tumor Necrosis Factor Mediated by Glucocorticoids?.
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Identification of monocyte chemotactic activity produced by malignant cells.
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Science 245, 1490-1493
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Induction of manganous superoxide dismutase by tumor necrosis factor: possible protective mechanism.
G. Wong and D. Goeddel (1988)
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Tumor Necrosis Factor in Middle Ear Effusions.
D. Ophir, T. Hahn, A. Schattner, D. Wallach, and A. Aviel (1988)
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Antiproliferative activity of a hybrid protein between interferon-gamma and tumor necrosis factor-beta.
G. Feng, P. Gray, H. Shepard, and M. Taylor (1988)
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Cachectin/TNF and IL-1 induced by glucose-modified proteins: role in normal tissue remodeling.
H Vlassara, M Brownlee, K. Manogue, C. Dinarello, and A Pasagian (1988)
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Lymphotoxin is an important T cell-derived growth factor for human B cells.
J. Kehrl, M Alvarez-Mon, G. Delsing, and A. Fauci (1987)
Science 238, 1144-1146
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Human beta 2 interferon and B-cell differentiation factor BSF-2 are identical.
P. Sehgal, L. May, I Tamm, and J Vilcek (1987)
Science 235, 731-732
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Tumor necrosis factor reduces c-myc expression and cooperates with interferon-gamma in HeLa cells.
A Yarden and A Kimchi (1986)
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Lymphokines and Monokines in Anti-cancer Therapy.
W. Fiers, P. Brouckaert, R. Devos, L. Fransen, G. Leroux-Roels, E. Remaut, P. Suffys, J. Tavernier, J. Van der Heyden, and F. Van Roy (1986)
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Tumor Necrosis Factors: Gene Structure and Biological Activities.
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Tandem Arrangement of Genes Coding for Tumor Necrosis Factor (TNF-{alpha}) and Lymphotoxin (TNF-{beta}) in the Human Genome.
S.A. Nedospasov, A.N. Shakhov, R.L. Turetskaya, V.A. Mett, M.M. Azizov, G.P. Georgiev, V.G. Korobko, V.N. Dobrynin, S.A. Filippov, N.S. Bystrov, et al. (1986)
Cold Spring Harb Symp Quant Biol 51, 611-624
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Interferon gamma (IFNgamma ) and Tumor Necrosis Factor alpha Synergism in ME-180 Cervical Cancer Cell Apoptosis and Necrosis. IFNgamma INHIBITS CYTOPROTECTIVE NF-kappa B THROUGH STAT1/IRF-1 PATHWAYS.
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