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Science 22 November 1985:
Vol. 230. no. 4728, pp. 906 - 911
DOI: 10.1126/science.2932798
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Articles

Science, Vol 230, Issue 4728, 906-911
Copyright © 1985 by American Association for the Advancement of Science

articles

Control of directionality in lambda site specific recombination

W Bushman, JF Thompson, L Vargas, and A Landy

The simple relation between the substrates and products of site-specific recombination raises questions about the control of directionality often observed in this class of DNA transactions. For bacteriophage lambda, viral integration and excision proceed by discrete pathways, and DNA substrates with the intrinsic property of recombining in only one direction can be constructed. These pathways display an asymmetric reliance on a complex array of protein binding sites, and they respond differently to changes in the concentrations of the relevant proteins. The Escherichia coli protein integration host factor (IHF) differentially affects integrative and excisive recombination, thereby influencing directionality. A four- to eightfold increase in intracellular IHF coincides with the transition from exponential to stationary phase; this provides a mechanism for growth phase-dependent regulation of recombination that makes the cellular physiology an intrinsic part of the recombination reaction.


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DNA Cloning Using In Vitro Site-Specific Recombination.
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Characterization of the mIHF Gene of Mycobacterium smegmatis.
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