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Science 15 November 1985:
Vol. 230. no. 4727, pp. 805 - 807
DOI: 10.1126/science.2997920
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Articles

Science, Vol 230, Issue 4727, 805-807
Copyright © 1985 by American Association for the Advancement of Science

articles

Expression of the Escherichia coli lacZ gene on a plasmid vector in a cyanobacterium

JS Buzby, RD Porter, and SE Stevens Jr

A biphasic plasmid vector was used to introduce the Escherichia coli K-12 lac operon into the unicellular cyanobacterium Agmenellum quadruplicatum PR-6. The PR-6 transformants expressed beta-galactosidase at nearly as high a level as did Escherichia coli transformants. In order to accomplish this, it was necessary to obtain PR-6 mutants that could be transformed by plasmids with unmodified recognition sites for the endogenous PR-6 restriction endonuclease Aqu I. These mutants were generated by a variation of the ectopic mutagenesis techniques that have been used in other naturally transforming bacteria. The ability to assay the expression of lacZ in PR-6 paves the way for the construction of gene fusions with various PR-6 promoters and quantitation of their expression under specific in vivo conditions.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
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J. Yan, G. Kurisu, and W. A. Cramer (2006)
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Functional Insensitivity of the Cytochrome b6f Complex to Structure Changes in the Hinge Region of the Rieske Iron-Sulfur Protein.
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CO2 Response Element and Corresponding trans-acting Factor of the Promoter for Ribulose-1,5-bisphosphate Carboxylase/oxygenase Genes in Synechococcus sp. PCC7002 Found by an Improved Electrophoretic Mobility Shift Assay.
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Assembly of Photosystem I. I. INACTIVATION OF THE rubA GENE ENCODING A MEMBRANE-ASSOCIATED RUBREDOXIN IN THE CYANOBACTERIUM SYNECHOCOCCUS SP. PCC 7002 CAUSES A LOSS OF PHOTOSYSTEM I ACTIVITY.
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