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Science 25 October 1985:
Vol. 230. no. 4724, pp. 442 - 445
DOI: 10.1126/science.2996134
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Articles

Science, Vol 230, Issue 4724, 442-445
Copyright © 1985 by American Association for the Advancement of Science

articles

Identification of the protein encoded by the E6 transforming gene of bovine papillomavirus

EJ Androphy, JT Schiller, and DR Lowy

Papillomaviruses (PV) contain several conserved genes that may encode nonstructural proteins; however, none of these predicted gene products have been identified. Papillomavirus E6 genes are retained and expressed as RNA in PV-associated human and animal carcinomas and cell lines. This suggests that the E6 gene product may be important in the maintenance of the malignant phenotype. The E6 open reading frame of the bovine papillomavirus (BPV) genome has been identified as one of two BPV genes that can independently transform mouse cells in vitro. A polypeptide encoded by this region of BPV was produced in a bacterial expression vector and used to raise antisera. The antisera specifically immunoprecipitated the predicted 15.5-kilodalton BPV E6 protein from cells transformed by the E6 gene. The E6 protein was identified in both the nuclear and membrane fractions of these transformed cells.


THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES:
DNA Supercoiling Factor Localizes to Puffs on Polytene Chromosomes in Drosophila melanogaster.
M. Kobayashi, N. Aita, S. Hayashi, K. Okada, T. Ohta, and S. Hirose (1998)
Mol. Cell. Biol. 18, 6737-6744
   Abstract »    Full Text »
Identification of an alpha  Helical Motif Sufficient for Association with Papillomavirus E6.
J. J. Chen, Y. Hong, E. Rustamzadeh, J. D. Baleja, and E. J. Androphy (1998)
J. Biol. Chem. 273, 13537-13544
   Abstract »    Full Text »    PDF »
Interaction of the Bovine Papillomavirus E6 Protein with the Clathrin Adaptor Complex AP-1.
X. Tong, W. Boll, T. Kirchhausen, and P. M. Howley (1998)
J. Virol. 72, 476-482
   Abstract »    Full Text »    PDF »
The Bovine Papillomavirus E6 Protein Binds to the LD Motif Repeats of Paxillin and Blocks Its Interaction with Vinculin and the Focal Adhesion Kinase.
X. Tong, R. Salgia, J.-L. Li, J. D. Griffin, and P. M. Howley (1997)
J. Biol. Chem. 272, 33373-33376
   Abstract »    Full Text »    PDF »
The bovine papillomavirus E6 oncoprotein interacts with paxillin and disrupts the actin cytoskeleton.
X. Tong and P. M. Howley (1997)
PNAS 94, 4412-4417
   Abstract »    Full Text »    PDF »
Interaction of papillomavirus E6 oncoproteins with a putative calcium-binding protein.
J. Chen, C. Reid, V Band, and E. Androphy (1995)
Science 269, 529-531
   Abstract »    PDF »
Transcription Factor E2 Regulates BPV-1 DNA Replication In Vitro by Direct Protein-Protein Interaction.
L. Yang, I. Mohr, R. Li, T. Nottoli, S. Sun, and M. Botchan (1991)
Cold Spring Harb Symp Quant Biol 56, 335-346
   Abstract »    PDF »
The E5 transforming gene of bovine papillomavirus encodes a small, hydrophobic polypeptide.
R Schlegel, M Wade-Glass, M. Rabson, and Y. Yang (1986)
Science 233, 464-467
   Abstract »    PDF »


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